Recombinant Mouse anti-Human KLF4 Monoclonal antibody (2F6)
CAT#: MOB-1026CT
Recombinant Mouse monoclonal antibody to Human KLF4, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/110_C2_1_selected.jpg
Normal Tissue
Figure 2 High expression in esophagus
Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_B_4_1.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Weak
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (ASC TERT1, hTCEpi, hTERT-HME1, OE19, U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136826-KLF4
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Specifications
- Immunogen
- Purified recombinant fragment of Human KLF4 expressed in E. Coli.
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- 2F6
- Applications
- Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Immunocytochemistry/Immunofluorescence; Immunohistochemistry paraffin embedded sections; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Ascites
- Format
- Liquid
- Buffer
- Preservative: 0.03% Sodium Azide Constituents: Ascites
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Target
- Alternative Names
- KLF4; Kruppel-like factor 4 (gut); EZF; GKLF; Krueppel-like factor 4; gut-enriched krueppel-like factor; epithelial zinc finger protein EZF; endothelial Kruppel-like zinc finger protein;
- Gene ID
- 9314
- UniProt ID
- O43474
- Protein Refseq
- NP_004226
- Function
- DNA binding; RNA polymerase II transcription factor binding; RNA polymerase II transcription factor binding; double-stranded DNA binding; metal ion binding; phosphatidylinositol 3-kinase regulator activity; sequence-specific DNA binding; sequence-specific
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "KLF4"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1956 | Hi-Affi™ Recombinant Rabbit Anti-KLF4 Monoclonal Antibody (DS1956AB) | ELISA, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0901-CN | Recombinant Rabbit Anti-KLF4 Monoclonal Antibody (JF98-08) | WB, IF, IHC | Rabbit IgG |
| ZG-0387J | Mouse Anti-KLF4 Recombinant Antibody (ZG-0387J) | WB, IHC, ELISA | Mouse IgG |
| ZG-0388J | Mouse Anti-KLF4 Recombinant Antibody (ZG-0388J) | WB, IHC, IF, ELISA | Mouse IgG |
| VS3-CJ33 | Mouse Anti-KLF4 Recombinant Antibody (VS3-CJ33) | WB, ELISA | Mouse IgG |
| VS3-XY980 | Mouse Anti-KLF4 Recombinant Antibody (clone 1E5) | ELISA, WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC96 | AbPlus™ Anti-KLF4 Magnetic Beads (VS-0724-YC96) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC36 | Mouse Anti-KLF4 Recombinant Antibody (VS-0924-YC36) - Cancer Stem Cell Marker | IHC, WB, IF | |
| VS-0924-YC63 | Rabbit Anti-KLF4 Antibody (VS-0924-YC63) - Cancer Stem Cell Marker | IHC, WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3795 | Anti-KLF4 Immunohistochemistry Kit | IHC |
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