Mouse Anti-KLHL21 Monoclonal Antibody (VS7-0425-WR234)
CAT#: VS7-0425-WR234
This product is a mouse IgG antibody that binds to human KLHL21. The antibody can be used for WB, IHC-P, IF, and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59307/1017_G5_2_selected.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51364/123614_A_8_3.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51364/123614_A_8_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51364/123614_A_7_5.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51364/123614_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162413-KLHL21
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Specifications
- Immunogen
- Recombinant human KLHL21 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human KLHL21
- Species Reactivity
- Human
- Molecule Class
- Kelch-Like Protein
- Applications
- WB, IHC-P, IF, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- Kelch Like Family Member 21; Kelch-Like 21 (Drosophila)
- Gene ID
- 9903
- UniProt ID
- Q9UJP4
- Long Name
- Kelch Like Family Member 21
- Cellular Localization
- Cytoplasm; Cytoskeleton
- Protein Refseq
- NP_001311238.1
- Function
- Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for efficient chromosome alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "KLHL21"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1032CT | Recombinant Mouse anti-Human KLHL21 Monoclonal antibody (3I5) | ELISA, IHC-P, WB | |
| ZG-0249C | Mouse Anti-KLHL21 Recombinant Antibody (ZG-0249C) | WB, IHC, ELISA | Mouse IgG |
| VS3-WK85 | Mouse Anti-KLHL21 Recombinant Antibody (clone F4-H4) | WB, ELISA | Mouse IgG1 |
| VS3-XY983 | Mouse Anti-KLHL21 Recombinant Antibody (clone 2H4) | ELISA, WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3801 | Anti-KLHL21 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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