Mouse Anti-KLK3 Recombinant Antibody (clone 1L123H-f)
CAT#: FAMAB-0159JF
Recombinant monoclonal antibody specifically binds to prostate-specific antigen (PSA). This mouse anti-PSA antibody (1L123H-f) can detect the fraction of PSA in human sera.
Published Data
Gene Expression
WB
Figure 1 VEGF-C activation by KLK3 is enhanced by CCBE1.
Activation of VEGF-C by KLK3 is enhanced by CCBE1.
Jha, S. K., Rauniyar, K., Chronowska, E., Mattonet, K., Maina, E. W., Koistinen, H., ... & Jeltsch, M. (2019). KLK3/PSA and cathepsin D activate VEGF-C and VEGF-D. Elife, 8, e44478.
ELISA
Figure 2 The receptor-activating properties of VEGF-C are differentially affected by cathepsin D cleavage.
Shown are the results of Ba/F3-VEGFR/EpoR assays used to evaluate the receptor-activating properties of cathepsin D- and KLK3- cleaved proteins.
Jha, S. K., Rauniyar, K., Chronowska, E., Mattonet, K., Maina, E. W., Koistinen, H., ... & Jeltsch, M. (2019). KLK3/PSA and cathepsin D activate VEGF-C and VEGF-D. Elife, 8, e44478.
IF
Figure 3 The KLK3- and cathepsin D-forms of VEGF-C induce lymphangiogenesis and angiogenesis in vivo.
Shown are the immunofluorescent stainings of the blood and lymphatic vessels in skeletal muscle.
Jha, S. K., Rauniyar, K., Chronowska, E., Mattonet, K., Maina, E. W., Koistinen, H., ... & Jeltsch, M. (2019). KLK3/PSA and cathepsin D activate VEGF-C and VEGF-D. Elife, 8, e44478.
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Normal Tissue
Figure 1 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/764/2093_A_1_5_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Group enriched (BEWO, NB-4, RPMI-8226, SH-SY5Y, U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000142515-KLK3
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Specifications
- Immunogen
- Human kallikrein related peptidase 3
- Host Species
- Mouse
- Derivation
- Mouse phage display scFv library
- Type
- Mouse IgG
- Specificity
- Human PSA
- Species Reactivity
- Human
- Clone
- 1L123H-f
- Applications
- WB, ELISA, IF
- Related Disease
- Prostatic diseases
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The KLK3 antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-603-S(P) | Recombinant Anti-human KLK3 Antibody scFv Fragment | WB, IHC, FuncS | scFv |
| MHH-603-S(P) | Recombinant Human Anti-human KLK3 Antibody scFv Fragment | FC, Neut, Biosensors, FuncS | scFv |
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| PSBL-678 | Mouse Anti-KLK3 Recombinant Antibody; scFv Fragment (PSBL-678) | WB, FuncS | Mouse scFv |
| FAMAB-0159JF-S(P) | Mouse Anti-KLK3 Recombinant Antibody (clone 1L123H-f); scFv Fragment | IF, ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-104 | Recombinant Anti-Human KLK3 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-603 | Recombinant Human Anti-human KLK3 Antibody | WB, Neut, FuncS | IgG |
| PABL-685 | Mouse Anti-KLK3 Recombinant Antibody (PABL-685) | WB, FuncS | Mouse IgG |
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| ZG-0969F | Mouse Anti-KLK3 Recombinant Antibody (clone 1A7) | ELISA, FC, ICC, IHC-Fr, IHC-P, WB | Mouse IgG1 |
| ZG-0980F | Mouse Anti-KLK3 Recombinant Antibody (clone 2B7) | ELISA, FC, ICC, IHC-Fr, IHC-P, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-603-F(E) | Recombinant Human Anti-human KLK3 Antibody Fab Fragment | FC, ELISA, FuncS | Fab |
| PFBL-256 | Mouse Anti-KLK3 Recombinant Antibody Fab Fragment (PFBL-256) | WB, IHC, FuncS | Mouse Fab |
| PFBL-678 | Mouse Anti-KLK3 Recombinant Antibody; Fab Fragment (PFBL-678) | WB, FuncS | Mouse Fab |
| HPAB-0619-WJ-F(E) | Mouse Anti-KLK3 Recombinant Antibody; Fab Fragment (HPAB-0619-WJ-F(E)) | ELISA | Mouse Fab |
| HPAB-0620-WJ-F(E) | Human Anti-KLK3 Recombinant Antibody; Fab Fragment (HPAB-0620-WJ-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0663MZ | Chicken Anti-Human prostate specific antigen (PSA) Polyclonal IgY | N/A | Chicken antibody |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1645CQ | Recombinant Mouse Anti-KLK3 Antibody (CBL579) | Neut | IgG2b |
| NEUT-1646CQ | Recombinant Mouse Anti-KLK3 Antibody (CBL129) | Neut, WB | IgG2 |
| NEUT-1647CQ | Recombinant Mouse Anti-KLK3 Antibody (5F28) | Neut, WB | IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1962 | Hi-Affi™ Recombinant Rabbit Anti-KLK3 Monoclonal Antibody (DS1962AB) | IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX648 | Mouse Anti-KLK3 Recombinant Antibody (clone 551CT16.3.5) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3805 | Anti-KLK3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3806 | Anti-Human KLK3 Immunohistochemistry Kit | IHC |
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