Mouse Anti-KLK3 Recombinant Antibody (clone 5A11E2)
CAT#: VS7-0425-WR696
This product is a mouse IgG1 antibody that binds to human KLK3. The antibody 5A11E2 recognizes specific amino acid domain of KLK3 (aa26-251). It can be used for IHC and FC assays.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/764/2093_A_1_5_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Group enriched (BEWO, NB-4, RPMI-8226, SH-SY5Y, U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000142515-KLK3
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Specifications
- Immunogen
- Recombinant KLK3 (aa26-251) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human KLK3
- Species Reactivity
- Human
- Clone
- 5A11E2
- Molecule Class
- Serine Protease Enzyme
- Applications
- IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- Kallikrein Related Peptidase 3; Gamma-Seminoprotein; P-30 Antigen; Kallikrein-3; Semenogelase; Seminin; APS; PSA
- Gene ID
- 354
- UniProt ID
- P07288
- Long Name
- Kallikrein Related Peptidase 3
- Cellular Localization
- Secreted
- Post Translation Modifications
- Disulfide bond; Glycoprotein; Zymogen
- Protein Refseq
- NP_001025218.1
- Function
- Hydrolase; Protease; Serine protease
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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