Mouse Anti-KRT10 Recombinant Antibody (clone 1C3D9)
CAT#: VS7-0425-WR249
This product is a mouse IgG1 antibody that binds to human KRT10. The antibody 1C3D9 recognizes specific amino acid domain of human KRT10 (AA: 345-454 ). It can be used for WB and IHC assays. It cross reacts with monkey.
Gene Expression
Normal Tissue
Figure 1 High expression in skin 1
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12014/ihc_selected.jpg
Normal Tissue
Figure 2 Low expression in colon
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12014/143516_A_8_3_val_selected.jpg
Normal Tissue
Figure 3 Skin
Cells in corneal layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in granular layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity:>75% Eccrine glands Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/132/2531_B_8_1.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enriched (hTCEpi)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186395-KRT10
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Specifications
- Immunogen
- Recombinant human KRT10 (AA: 345-454 ) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human KRT10
- Species Reactivity
- Human, Monkey
- Clone
- 1C3D9
- Molecule Class
- Type I Intermediate Filament Protein
- Applications
- WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Epidermolytic hyperkeratosis 2A (EHK2A); Epidermolytic hyperkeratosis 2B, autosomal recessive (EHK2B); Ichthyosis, annular epidermolytic, 1 (AEI1); Ichthyosis with confetti (IWC); Ichthyosis histrix, Lambert type (IHL)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- BIE; EHK; IHL; K10; KPP; BCIE; CK10; EHK2; EHK2A; EHK2B
- Gene ID
- 3858
- UniProt ID
- P13645
- Long Name
- Keratin 10
- Cellular Localization
- Cytoplasm; Intermediate filament; Keratin; Secreted
- Post Translation Modifications
- Disulfide bond; Phosphoprotein
- Protein Refseq
- NP_000412.4
- Function
- Plays a role in the establishment of the epidermal barrier on plantar skin (By similarity).; Involved in the maintenance of cell layer development and keratin filament bundles in suprabasal cells of the epithelium (By similarity).; (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization.; (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "KRT10"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1975 | Hi-Affi™ Recombinant Rabbit Anti-KRT10 Monoclonal Antibody (DS1975AB) | ICC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0434-CN | Recombinant Rabbit Anti-KRT10 Monoclonal Antibody (CBACN-174) | WB, IF, IHC | Rabbit IgG |
| ZG-0230J | Mouse Anti-KRT10 Recombinant Antibody (ZG-0230J) | IHC-P, IF | Mouse IgG |
| ZG-0231J | Mouse Anti-KRT10 Recombinant Antibody (ZG-0231J) | IHC-P, IF | Mouse IgG |
| ZG-1087F | Mouse Anti-KRT10 Recombinant Antibody (clone RKSE60) | FC, ICC, IHC-Fr, WB | Mouse IgG1 |
| VS1-YJ41 | Mouse Anti-KRT10 Recombinant Antibody (clone AE20) | IHC-P, IHC-Fr, WB | Mouse IgG |
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