Mouse Anti-KRT10 Recombinant Antibody (clone 3C2F5) (CAT#: VS7-0425-WR248)
This product is a mouse IgG1 antibody that binds to human KRT10. The antibody 3C2F5 recognizes specific amino acid domain of human KRT10 (AA: 345-454 ). It can be used for WB, IHC, and FC assays. It cross reacts with mouse, monkey, and rat.
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Normal Tissue
RNA Expression
Figure 1 High expression in skin 1
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12014/ihc_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12014/ihc_selected.jpg
Figure 2 Low expression in colon
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12014/143516_A_8_3_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12014/143516_A_8_3_val_selected.jpg
Figure 3 Skin
(Cells in corneal layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in granular layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity:>75% Eccrine glands Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/132/2531_B_8_1.jpg
(Cells in corneal layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in granular layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity:>75% Eccrine glands Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/132/2531_B_8_1.jpg
Figure 4 RNA cell line category: Cell line enriched (hTCEpi)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186395-KRT10
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186395-KRT10
Specifications
- Immunogen
- Recombinant human KRT10 (AA: 345-454 ) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human KRT10
- Species Reactivity
- Human, Mouse, Monkey, Rat
- Clone
- 3C2F5
- Molecule Class
- Type I Intermediate Filament Protein
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated
- Related Disease
- Epidermolytic hyperkeratosis 2A (EHK2A); Epidermolytic hyperkeratosis 2B, autosomal recessive (EHK2B); Ichthyosis, annular epidermolytic, 1 (AEI1); Ichthyosis with confetti (IWC); Ichthyosis histrix, Lambert type (IHL)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- BIE; EHK; IHL; K10; KPP; BCIE; CK10; EHK2; EHK2A; EHK2B
- Gene ID
- 3858
- UniProt ID
- P13645
- Long Name
- Keratin 10
- Cellular Localization
- Cytoplasm; Intermediate filament; Keratin; Secreted
- Post Translation Modifications
- Disulfide bond; Phosphoprotein
- Protein Refseq
- NP_000412.4
- Function
- Plays a role in the establishment of the epidermal barrier on plantar skin (By similarity).; Involved in the maintenance of cell layer development and keratin filament bundles in suprabasal cells of the epithelium (By similarity).; (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization.; (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur.
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1975 | Hi-Affi™ Recombinant Rabbit Anti-KRT10 Monoclonal Antibody (DS1975AB) | ICC, IHC-P, IP, WB | IgG |
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