Rabbit Anti-KRT14 Recombinant Antibody (clone R02-7I6)
CAT#: VS3-XY3410
It is a recombinant rabbit antibody targeting the KRT14 (Keratin 14). The antibody specifically reacts with human KRT14. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry and Immunofluorescence is 1/500-1/1000, 1/50-1/100, 1/20, 1/50-1/100 and 1/50-1/200, respectively.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23040/1660_C10_5_selected.jpg
Normal Tissue
Figure 2 Skin 1
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23040/51237_B_9_1_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23040/51237_B_8_5.jpg
Normal Tissue
Figure 4 Skin
Keratinocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Langerhans Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Melanocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/134/1050_B_7_1.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HaCaT, HBEC3-KT, hTCEpi, hTERT-HME1, U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000186847-KRT14
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human KRT14
- Species Reactivity
- Human
- Clone
- R02-7I6
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Dermatopathia Pigmentosa Reticularis
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- K14; NFJ; CK14; EBS1; EBS3; EBS4; EBS1A; EBS1B; EBS1C; EBS1D
- Gene ID
- 3861
- UniProt ID
- P02533
- Long Name
- Keratin 14
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm, Intermediate filament, Keratin, Nucleus
- Post Translation Modifications
- A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus.
Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex.
- Protein Refseq
- NP_000517.2
- Function
- The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "KRT14"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-1120MZ | Chicken Anti-Keratin 14 Polyclonal IgY | IHC | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1978 | Hi-Affi™ Recombinant Rabbit Anti-KRT14 Monoclonal Antibody (DS1978AB) | IHC-P, IHC-Fr | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0597F | Mouse Anti-KRT14 Recombinant Antibody (clone LL002) | IHC-Fr, IHC-P | Mouse IgG3 |
| ZG-0682F | Mouse Anti-KRT14 Recombinant Antibody (clone 16.4) | IHC-Fr, WB | Mouse IgG2a |
| VS3-FY809 | Recombinant Mouse Anti-KRT14 Antibody (clone 1A2H8) | ELISA, WB, IHC, ICC, FC | Mouse IgG2a |
| VS3-FY810 | Recombinant Mouse Anti-KRT14 Antibody (clone 3A1F6) | ELISA, WB, IHC, ICC, FC | Mouse IgG1 |
| VS3-FY811 | Recombinant Mouse Anti-KRT14 Antibody (clone 4D2A5) | ELISA, IHC, ICC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1380 | AbPlus™ Anti-KRT14 Magnetic Beads (VS-0724-YC1380) | IP, Protein Purification | |
| VS-0724-YC1431 | AbPlus™ Anti-Krt14 Magnetic Beads (VS-0724-YC1431) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1209 | Anti-KRT14 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3843 | Anti-Mouse KRT14 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3842 | Anti-Human KRT14 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3844 | Anti-Rat KRT14 Immunohistochemistry Kit | IHC |
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