Mouse Anti-KRT18 Recombinant Antibody (clone PT1876)
CAT#: VS3-CJ422
This product is a mouse antibody that recognizes human KRT18.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows distinct membranous and cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/21_G9_1_blue_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/22_G9_1_blue_red_green.jpg
Normal Tissue
Figure 5 Colon
Endocrine cells
Staining:
High
Intensity: Strong
Enterocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Collecting ducts
Staining:
High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_7_5.jpg
Normal Tissue
Figure 8 Placenta
Cytotrophoblasts
Staining:
High
Intensity: Strong
Quantity:>75%
Endothelial cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Hofbauer cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:
High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:
High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_3_7.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (A549, BEWO, CACO-2, CAPAN-2, MCF7, SK-BR-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000111057-KRT18
❮
❯
Specifications
- Immunogen
- Synthesized peptide derived from human Cytokeratin 18
- Host Species
- Mouse
- Type
- Mouse IgM, κ
- Specificity
- Human KRT18
- Species Reactivity
- Human
- Clone
- PT1876
- Applications
- WB, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:2000), Immunohistochemistry-Parraffin (1:100-1:500).
Target
- Alternative Names
- K18; CK-18; CYK18
- Gene ID
- 3875
- UniProt ID
- P05783
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm, Intermediate filament, Keratin, Nucleus
- Post Translation Modifications
- Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.
- Protein Refseq
- NP_000215.1; NP_954657.1
- Function
- Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| ZG-0462F | Mouse Anti-KRT18 Recombinant Antibody (ZG-0462F) | WB, IHC, ELISA | Mouse IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0318MZ | Chicken Anti-KRT18 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0408-YC-S(P) | Mouse Anti-KRT18 Recombinant Antibody; scFv Fragment (HPAB-0408-YC-S(P)) | WB | Mouse scFv |
| HPAB-0299-FY-S(P) | Mouse Anti-KRT18 Recombinant Antibody (clone CBLFY07); scFv Fragment | FC, WB, IF, IHC, IP | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0408-YC-F(E) | Mouse Anti-KRT18 Recombinant Antibody; Fab Fragment (HPAB-0408-YC-F(E)) | WB | Mouse Fab |
| HPAB-0299-FY-F(E) | Mouse Anti-KRT18 Recombinant Antibody (clone CBLFY07); Fab Fragment | FC, WB, IF, IHC, IP | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0159 | APC-A*02:01/Human K18 (LIYGTSSRA) MHC Tetramer | FCM | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC921 | AbPlus™ Anti-KRT18 Magnetic Beads (VS-0724-YC921) | IP, Protein Purification | |
| VS-0724-YC1035 | AbPlus™ Anti-KRT18 Magnetic Beads (VS-0724-YC1035) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY154 | Mouse Anti-NHP KRT18 Recombinant Antibody (clone C-04 (Ks18.04)) | WB, IHC, IF, FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC634 | CytoStream™ Rabbit Anti-KRT18 Polyclonal Antibody (VS13-YC634) | WB, ELISA, IHC-P, IHC-Fr, FC, ICC, IF | Rabbit IgG |
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