CytoStream™ Rabbit Anti-KRT18 Polyclonal Antibody (VS13-YC634)
CAT#: VS13-YC634
The CytoStream™ Rabbit Anti-KRT18 Polyclonal Antibody is specific for human, mouse and rat KRT18. KRT18 encodes keratin 18, forming intermediate filaments with keratin 8 in epithelium, affecting structural integrity. Mutations relate to cryptogenic cirrhosis, exhibiting two transcript variants for the same protein. The anti-KRT18 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1 μg per 1x 10⁶ cells).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows distinct membranous and cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/21_G9_1_blue_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/22_G9_1_blue_red_green.jpg
Normal Tissue
Figure 5 Colon
Endocrine cells
Staining:
High
Intensity: Strong
Enterocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Collecting ducts
Staining:
High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_7_5.jpg
Normal Tissue
Figure 8 Placenta
Cytotrophoblasts
Staining:
High
Intensity: Strong
Quantity:>75%
Endothelial cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Hofbauer cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:
High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:
High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_3_7.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (A549, BEWO, CACO-2, CAPAN-2, MCF7, SK-BR-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000111057-KRT18
❮
❯
Specifications
- Immunogen
- Recombinant human CK18 (aa 53-150) conjugated with KLH.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat KRT18
- Species Reactivity
- Human, Mouse, Rat, Dog (Predicted), Pig (Predicted), Cow (Predicted), Horse (Predicted), Rabbit (Predicted)
- Applications
- WB, ELISA, IHC-P, IHC-Fr, FC, ICC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01× TBS (pH 7.4) containing 50% Glycerol and 1% BSA.
- Preservative
- 0.03% Proclin 300
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
ELISA: 1/5000-1/10000
Immunohistochemistry-paraffin (IHC-P): 1/100-1/500
Immunohistochemistry-frozen (IHC-Fr): 1/100-1/500
Flow cytometry (FC): 1 μg per 1x 10⁶ cells
Immunocytochemistry (ICC): 1/100
Immunofluorescence (IF): 1/100-1/500
Target
- Alternative Names
- Keratin 18; Cell Proliferation-Inducing Gene 46 Protein; Keratin 18, Type I; CK-18; CYK18; K18;
- Gene ID
- 3875
- UniProt ID
- P05783
- Long Name
- Keratin 18
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Nucleus; Nucleolus; Cytoplasm; Perinuclear regio
- Protein Refseq
- NP_000215.1; NP_954657.1
- Function
- Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "KRT18"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1264z | Mouse Anti-KRT18 Recombinant Antibody (clone 21C5) | FC, IF, IHC, WB | Mouse IgG1 |
| HPAB-0408-YC | Mouse Anti-KRT18 Recombinant Antibody (HPAB-0408-YC) | WB, ELISA | Mouse IgG |
| ZG-0241J | Mouse Anti-KRT18 Recombinant Antibody (ZG-0241J) | IHC-P, IF, ICC | Mouse IgG |
| ZG-0242J | Mouse Anti-KRT18 Recombinant Antibody (ZG-0242J) | IHC-P, IF | Mouse IgG |
| ZG-0243J | Mouse Anti-KRT18 Recombinant Antibody (clone 1B3) | WB, IF, ICC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0318MZ | Chicken Anti-KRT18 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1982 | Hi-Affi™ Recombinant Rabbit Anti-KRT18 Monoclonal Antibody (DS1982AB) | IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0408-YC-S(P) | Mouse Anti-KRT18 Recombinant Antibody; scFv Fragment (HPAB-0408-YC-S(P)) | WB | Mouse scFv |
| HPAB-0299-FY-S(P) | Mouse Anti-KRT18 Recombinant Antibody (clone CBLFY07); scFv Fragment | FC, WB, IF, IHC, IP | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0408-YC-F(E) | Mouse Anti-KRT18 Recombinant Antibody; Fab Fragment (HPAB-0408-YC-F(E)) | WB | Mouse Fab |
| HPAB-0299-FY-F(E) | Mouse Anti-KRT18 Recombinant Antibody (clone CBLFY07); Fab Fragment | FC, WB, IF, IHC, IP | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0159 | APC-A*02:01/Human K18 (LIYGTSSRA) MHC Tetramer | FCM | |
| MHC-CN0160 | PE-A*02:01/Human K18 (LIYGTSSRA) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ422 | Mouse Anti-KRT18 Recombinant Antibody (clone PT1876) | WB, IHC-P | Mouse IgM, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC921 | AbPlus™ Anti-KRT18 Magnetic Beads (VS-0724-YC921) | IP, Protein Purification | |
| VS-0724-YC1035 | AbPlus™ Anti-KRT18 Magnetic Beads (VS-0724-YC1035) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY154 | Mouse Anti-NHP KRT18 Recombinant Antibody (clone C-04 (Ks18.04)) | WB, IHC, IF, FC | Mouse IgG1, kappa |
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