Mouse Anti-NHP KRT18 Recombinant Antibody (clone C-04 (Ks18.04))

Name: Mouse Anti-NHP KRT18 Recombinant Antibody (clone C-04 (Ks18.04))
SKU: VS-1024-XY154
Rating: 5 (1 reviews)

CAT#: VS-1024-XY154

The monoclonal antibody, derived from mouse, specifically recognizes KRT18 and shows reactivity across goat, hamster, human, mouse, non-human primate, and sheep. Utilizing a precisely engineered antigen, it ensures accurate detection in a variety of species. Proven effective in Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry (FC), this antibody offers exceptional versatility for analyzing KRT18 expression in diverse research and clinical contexts.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/if_selected.jpg

Subcellular Location Figure 2 IHC staining of human placenta

Immunohistochemical staining of human placenta shows distinct membranous and cytoplasmic positivity in trophoblastic cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/ihc_selected.jpg

Subcellular Location Figure 3 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/21_G9_1_blue_red_green.jpg

Subcellular Location Figure 4 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/22_G9_1_blue_red_green.jpg

Normal Tissue Figure 5 Colon

Endocrine cells
Staining:

High

Intensity: Strong

Enterocytes
Staining:

High

Intensity: Strong
Quantity:>75%

Goblet cells
Staining:

High

Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_8_3.jpg

Normal Tissue Figure 6 Liver

Cholangiocytes
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_9_4.jpg

Normal Tissue Figure 7 Kidney

Collecting ducts
Staining:

High

Intensity: Strong
Quantity:>75%
Distal tubules
Staining:

High

Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_7_5.jpg

Normal Tissue Figure 8 Placenta

Cytotrophoblasts
Staining:

High

Intensity: Strong
Quantity:>75%
Endothelial cells
Staining:

High

Intensity: Strong
Quantity: 75%-25%
Hofbauer cells
Staining:

High

Intensity: Strong
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:

High

Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:

High

Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_3_7.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enhanced (A549, BEWO, CACO-2, CAPAN-2, MCF7, SK-BR-3)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000111057-KRT18

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Specifications

Immunogen
Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431

Host Species
Mouse

Type
Mouse IgG1, kappa

Specificity
KRT18

Species Reactivity
Goat, Hamster, Human, Mouse, Non-human primate, Sheep

Clone
C-04 (Ks18.04)

Applications
WB, IHC, IF, FC

Product Property

Purification
Protein A purified

Purity
>95%, determined by SDS-PAGE

Format
Liquid

Buffer
PBS, pH 7.4, with 0.05% BSA

Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Applications

Application Notes
WB: 1-2 µg/mL, IHC: 1-2 µg/mL, IF: 1-2 µg/mL, FC: 1-2 µg/10⁶ cells

Target

Alternative Names
Keratin 18; Cell Proliferation-Inducing Gene 46 Protein; Keratin 18; Type I; CK-18; CYK18; K18

Gene ID
3875

UniProt ID
P05783

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-1024-XY154.pdf

MSDS

MSDS - VS-1024-XY154.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Recommended Dilution Buffer

CAT
Product Name

Isotype Control

CAT
Product Name

Secondary Antibody

CAT
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