Mouse Anti-KRT20 Recombinant Antibody (VS3-FY2758)
CAT#: VS3-FY2758
This extraordinary mouse-derived recombinant monoclonal antibody showcases a remarkable capacity to efficiently bind to KRT20. The calculated molecular weight of the protein is 48.5 kDa. It shows capability of cross-reacting with human. This monoclonal antibody can be used in Immunohistochemistry, ELISA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24684/if_selected.jpg
Normal Tissue
Figure 2 High expression in small intestine
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24309/54018_A_5_2_val_selected.jpg
Normal Tissue
Figure 3 Low expression in liver
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24309/54018_A_7_4_val_selected.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Gradient Gradient: Ascending Enterocytes - Microvilli Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32/127_A_8_3.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (NB-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171431-KRT20
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Keratin 20 of human
- Species Reactivity
- Human
- Applications
- IHC, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 48.5 kDa.
- Related Disease
- Cancer
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- Keratin 20; Keratin 20, Type I; Protein IT; CK-20; K20; Keratin, Type I Cytoskeletal 20; Cytokeratin 20
- Gene ID
- 54474
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm, Intermediate filament, Keratin
- Post Translation Modifications
- Hyperphosphorylation at Ser-13 oCcurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity).
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
- Protein Refseq
- NP_061883.1
- Function
- Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "KRT20"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1985 | Hi-Affi™ Recombinant Rabbit Anti-KRT20 Monoclonal Antibody (DS1985AB) | IHC-Fr, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0248J | Mouse Anti-KRT20 Recombinant Antibody (ZG-0248J) | IHC-P, IF | Mouse IgG |
| ZG-0325U | Rabbit Anti-KRT20 Recombinant Antibody (clone E19-I) | IHC-P | Rabbit IgG |
| VS3-FY825 | Recombinant Mouse Anti-KRT20 Antibody (clone 10B5-4H7-5A9) | IHC-P | Mouse IgG2a |
| VS11-QX93 | Mouse Anti-KRT20 Recombinant Antibody (clone 10A2) | ELISA, WB, IHC | Mouse IgG2a, κ |
| VS11-QX94 | Mouse Anti-KRT20 Recombinant Antibody (clone 10F6B12A9) | ELISA, WB, IHC | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1216 | Anti-KRT20 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3867 | Anti-Rat KRT20 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3866 | Anti-Human KRT20 Immunohistochemistry Kit | IHC |
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