Mouse Anti-KRT9 Recombinant Antibody (clone 8C2A6)
CAT#: VS7-0425-WR275
This product is a mouse IgG2b antibody that binds to human KRT9. The antibody 8C2A6 recognizes specific amino acid domain of human KRT9 (AA: 153-460). It can be used for WB, IHC, and FC assays.
Gene Expression
Normal Tissue
Figure 1 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37322/163025_A_9_8.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (BEWO, HaCaT, U-2197, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171403-KRT9
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Specifications
- Immunogen
- Recombinant human KRT9 (AA: 153-460) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human KRT9
- Species Reactivity
- Human
- Clone
- 8C2A6
- Molecule Class
- Type II Cytokeratin Protein
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated
- Related Disease
- Palmoplantar keratoderma, epidermolytic, 1 (EPPK1)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- KRT9; Type I Cytoskeletal 9; Cytokeratin-9; Keratin 9; Epidermolytic Palmoplantar Keratoderma; K9; Keratin 9, Type I; Keratin-9; Cytokeratin 9; CK-9; EPPK
- Gene ID
- 3857
- UniProt ID
- P35527
- Long Name
- Keratin 9
- Cellular Localization
- Intermediate filament; Keratin
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_000217.2
- Function
- May serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues. Plays a role in keratin filament assembly.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "KRT9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0533CT | Recombinant Mouse anti-Human KRT9 Monoclonal antibody (Lt0.80/Lt0.327) | ICC, IF, IHC-Fr, IHC-P, WB | |
| MRO-0454-CN | Recombinant Rabbit Anti-KRT9 Monoclonal Antibody (CBACN-190) | WB, IHC, IP | Rabbit IgG |
| VS3-FY831 | Recombinant Rabbit Anti-KRT9 Antibody (clone R04-6B7) | WB, IHC-P, IP | Rabbit IgG |
| VS7-0425-WR273 | Mouse Anti-KRT9 Recombinant Antibody (clone 4H3C2) | WB, FC | Mouse IgG2b |
| VS7-0425-WR274 | Mouse Anti-KRT9 Recombinant Antibody (clone 4B3E1) | WB, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1995 | Hi-Affi™ Recombinant Rabbit Anti-KRT9 Monoclonal Antibody (DS1995AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3901 | Anti-KRT9 Immunohistochemistry Kit | IHC |
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