Rabbit Anti-KSR1 Recombinant Antibody (clone R05-4F7)
CAT#: VS3-XY3414
The product is a recombinant rabbit antibody specific for KSR1 (Kinase suppressor of ras 1), which promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP. It recognizes human KSR1 and it also has cross reactivity against mouse KSR1. This product has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HDLM-2
Immunofluorescent staining of human cell line HDLM-2 shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11878/1962_C10_4_selected.jpg
Normal Tissue
Figure 2 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11215/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9623/33213_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9623/33213_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9623/33213_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9623/33213_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9623/33213_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9623/33213_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HDLM-2, HEL, HMC-1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141068-KSR1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse KSR1
- Species Reactivity
- Human, Mouse
- Clone
- R05-4F7
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Breast Adenocarcinoma; Rasopathy
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- KSR; RSU2
- Gene ID
- 8844
- UniProt ID
- Q8IVT5
- Long Name
- Kinase suppressor of ras 1
- Cellular Localization
- Cytoplasm, Cell membrane, Cell projection
- Post Translation Modifications
- Phosphorylated on Ser-311 and, to a higher extent, on Ser-406 by MARK3.
Dephosphorylated on Ser-406 by PPP2CA.
In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated.
Phosphorylated by PKA at Ser-888.
Phosphorylation at Ser-888 is required for cAMP-dependent activation of MAPK1 and/or MAPK3.
- Protein Refseq
- XP_011523731.1
- Function
- Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases (By similarity).
Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2 (PubMed:29433126).
Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "KSR1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2303CT | Recombinant Mouse anti-Human KSR1 Monoclonal antibody (N200) | ELISA, IP, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1998 | Hi-Affi™ Recombinant Rabbit Anti-KSR1 Monoclonal Antibody (DS1998AB) | ICC, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3902 | Anti-KSR1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3903 | Anti-Mouse KSR1 Immunohistochemistry Kit | IHC |
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