Mouse Anti-LacZ Recombinant Antibody (clone 3B7D5) (CAT#: ZG-0244U)

Figure 2 Mouse Anti-LacZ Antibody (ZG-0244U) in IF

Immunofluorescence staining of 293T cells transfected with ZG-0244U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). The image on the right is the 293T cells transfected without lacZ antibody

Figure 3 Mouse Anti-LacZ Antibody (ZG-0244U) in FC

Overlay histogram showing 293T transfected cells (red line) or untransfected cells (green line) stained with ZG-0244U at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C.

Figure 4 Mouse Anti-LacZ Antibody (ZG-0244U) in IP

Immunoprecipitating lacZ in 293T transfected whole cell lysate Lane 1: Mouse control IgG in 293T transfected whole cell lysate
Lane 2: ZG-0244U (2µl) + 293T transfected whole cell lysate (500µg)
Lane 3: 293T transfected whole cell lysate (20µg)
For western blotting, the blot was detected with ZG-0244U at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000

Figure 1 Mouse Anti-LacZ Antibody (ZG-0244U) in WB

Lane 1: Mouse control IgG in 293T transfected whole cell lysate
Lane 2: ZG-0244U (2 µL) + 293T transfected whole cell lysate (500 µg)
Lane 3: 293T transfected whole cell lysate (20 µg)
For western blotti ng, the blot was detected with ZG-0244U at 1: 2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1: 2000