Rabbit Anti-LDHA Recombinant Antibody (VS13-YC650)
CAT#: VS13-YC650
The Rabbit Anti-LDHA Recombinant Antibody is specific for human, mouse and rat LDHA. LDHA encodes an enzyme converting pyruvate to lactate in anaerobic glycolysis, found in muscle tissues, linked to exertional myoglobinuria, and crucial in cancer-associated glycolytic shifts. The anti-LDHA antibody can be used for Western blot (1/500-1/1000), Immunohistochemistry (1/50-1/100), Immunofluorescence (1/50-1/200), Immunoprecipitation (1/20).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15336/962_G3_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human skeletal muscle
Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15336/35308_B_3_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69404/153900_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69404/153900_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69404/153900_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69404/153900_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69404/153900_A_5_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69404/153900_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134333-LDHA
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Specifications
- Immunogen
- Recombinant human lactate dehydrogenase.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat LDHA
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-Fr, IHC-P, ICC, IF, IP
- Conjugate
- NONE
- Related Disease
- Fanconi-Bickel Syndrome and Myoglobinuria
Product Property
- Clonality
- Monoclonal
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Immunoprecipitation (IP): 1/20
Target
- Alternative Names
- Lactate Dehydrogenase A; Cell Proliferation-Inducing Gene 19 Protein; Renal Carcinoma Antigen NY-REN-59; LDH Muscle Subunit; LDH-A; LDH-M; Epididymis Secretory Sperm Binding Protein Li 133P; L-Lactate Dehydrogenase A Chain;Proliferation-Inducing Gene 19; Lactate Dehydrogenase M; EC 1.1.1.27; HEL-S-133P; GSD11; PIG19; LDHM;
- Gene ID
- 3939
- UniProt ID
- P00338
- Long Name
- Lactate Dehydrogenase A
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "LDHA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1952z | Mouse Anti-LDHA Recombinant Antibody (clone 8C6) | WB, IHC, IP | Mouse IgG1 |
| MOB-1076CT | Recombinant Mouse anti-Human LDHA Monoclonal antibody (30) | ELISA, WB | |
| MRO-0921-CN | Recombinant Mouse Anti-LDHA Monoclonal Antibody (20-179) | WB, IHC | Mouse IgG1 |
| VS3-CJ201 | Rabbit Anti-LDHA Recombinant Antibody (VS3-CJ201) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS-0923-FY106 | Recombinant Mouse Anti-LDHA Antibody (VS-0923-FY106) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2029 | Hi-Affi™ Recombinant Rabbit Anti-LDHA Monoclonal Antibody (DS2029AB) | FC, ICC, IF, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC910 | AbPlus™ Anti-LDHA Magnetic Beads (VS-0724-YC910) | IP, Protein Purification | |
| VS-0724-YC1052 | AbPlus™ Anti-Ldha Magnetic Beads (VS-0724-YC1052) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3971 | Anti-LDHA Immunohistochemistry Kit | IHC | |
| VS-0525-XY3972 | Anti-Mouse LDHA Immunohistochemistry Kit | IHC |
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