Rabbit Anti-LDHA Recombinant Antibody (VS13-YC650) (CAT#: VS13-YC650)
The Rabbit Anti-LDHA Recombinant Antibody is specific for human, mouse and rat LDHA. LDHA encodes an enzyme converting pyruvate to lactate in anaerobic glycolysis, found in muscle tissues, linked to exertional myoglobinuria, and crucial in cancer-associated glycolytic shifts. The anti-LDHA antibody can be used for Western blot (1/500-1/1000), Immunohistochemistry (1/50-1/100), Immunofluorescence (1/50-1/200), Immunoprecipitation (1/20).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15336/962_G3_2_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15336/962_G3_2_selected.jpg
Figure 2 IHC staining of human skeletal muscle
(Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15336/35308_B_3_6_selected.jpg
(Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15336/35308_B_3_6_selected.jpg
Figure 3 Cerebral cortex
(Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69404/153900_B_9_5.jpg
(Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69404/153900_B_9_5.jpg
Figure 4 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69404/153900_A_9_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69404/153900_A_9_3.jpg
Figure 5 Liver
(Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69404/153900_A_7_4.jpg
(Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69404/153900_A_7_4.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69404/153900_A_7_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69404/153900_A_7_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69404/153900_A_5_6.jpg
(Cells in seminiferous ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69404/153900_A_5_6.jpg
Figure 8 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69404/153900_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69404/153900_A_8_8.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134333-LDHA
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134333-LDHA
Specifications
- Immunogen
- Recombinant human lactate dehydrogenase.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat LDHA
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-Fr, IHC-P, ICC, IF, IP
- Conjugate
- NONE
- Related Disease
- Fanconi-Bickel Syndrome and Myoglobinuria
Product Property
- Clonality
- Monoclonal
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Immunoprecipitation (IP): 1/20
Target
- Alternative Names
- Lactate Dehydrogenase A; Cell Proliferation-Inducing Gene 19 Protein; Renal Carcinoma Antigen NY-REN-59; LDH Muscle Subunit; LDH-A; LDH-M; Epididymis Secretory Sperm Binding Protein Li 133P; L-Lactate Dehydrogenase A Chain;Proliferation-Inducing Gene 19; Lactate Dehydrogenase M; EC 1.1.1.27; HEL-S-133P; GSD11; PIG19; LDHM;
- Gene ID
- 3939
- UniProt ID
- P00338
- Long Name
- Lactate Dehydrogenase A
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "LDHA"
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1952z | Mouse Anti-LDHA Recombinant Antibody (clone 8C6) | WB, IHC, IP | Mouse IgG1 |
| MRO-0921-CN | Recombinant Mouse Anti-LDHA Monoclonal Antibody (20-179) | WB, IHC | Mouse IgG1 |
| MRO-2009-CN | Rabbit Anti-LDHA Polyclonal Antibody (MRO-2009-CN) | WB, IHC, FC | Rabbit IgG |
| VS3-CJ201 | Rabbit Anti-LDHA Recombinant Antibody (VS3-CJ201) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-FY844 | Recombinant Rabbit Anti-LDHA Antibody (clone R06-6J2) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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