Human Anti-LDL Recombinant Antibody (clone IK17) (CAT#: FAMAB-0056JF)
The recombinant human monoclonal antibody specifically binds to LDL. It is able to bind to MDA-LDL, intact OxLDL, OxLDL lipid and protein moieties but not to those of native LDL. This anti-LDL antibody IK17 can block the uptake of OxLDL by macrophages.
Specific Inquiry
IHC
Figure 1 Immunohistochemistry in human atherosclerotic lesion.
The localization of MPO-oxidized LDL in human atherosclerotic plaque was studied by immunohistochemistry in aortic.
Moguilevsky, N., Boudjeltia, K. Z., Babar, S., Delrée, P., Legssyer, I., Carpentier, Y., ... & Ducobu, J. (2004). Monoclonal antibodies against LDL progressively oxidized by myeloperoxidase react with ApoB-100 protein moiety and human atherosclerotic lesions. Biochemical and biophysical research communications, 323(4), 1223-1228.
WB
Figure 2 Study of the epitope. Identification of the smallest immunoreactive fragment by Western blotting.
This fragment of molecular weight 66 kDa, carrying the epitope reacting with the antibody.
Moguilevsky, N., Boudjeltia, K. Z., Babar, S., Delrée, P., Legssyer, I., Carpentier, Y., ... & Ducobu, J. (2004). Monoclonal antibodies against LDL progressively oxidized by myeloperoxidase react with ApoB-100 protein moiety and human atherosclerotic lesions. Biochemical and biophysical research communications, 323(4), 1223-1228.
ELISA
Figure 3 Recognition of human oxidized LDL.
The antibodies were tested by indirect ELISA experiments using native and MPO-oxidized human LDL.
Moguilevsky, N., Boudjeltia, K. Z., Babar, S., Delrée, P., Legssyer, I., Carpentier, Y., ... & Ducobu, J. (2004). Monoclonal antibodies against LDL progressively oxidized by myeloperoxidase react with ApoB-100 protein moiety and human atherosclerotic lesions. Biochemical and biophysical research communications, 323(4), 1223-1228.
Inhib
Figure 4 Specificity of the epitope recognized by the antibodies.
I-labeled antibody mixed with various amounts of cold purified antibodies diluted.
Moguilevsky, N., Boudjeltia, K. Z., Babar, S., Delrée, P., Legssyer, I., Carpentier, Y., ... & Ducobu, J. (2004). Monoclonal antibodies against LDL progressively oxidized by myeloperoxidase react with ApoB-100 protein moiety and human atherosclerotic lesions. Biochemical and biophysical research communications, 323(4), 1223-1228.
Specifications
- Host Species
- Human
- Derivation
- Human phage display Fab library
- Type
- Human IgG
- Specificity
- Human LDL, rabbit LDL
- Species Reactivity
- Human, Rabbit
- Clone
- IK17
- Applications
- WB, ELISA, IHC, Inhib
- Related Disease
- Atherosclerotic lesions
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The LDL antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Inhibition.
Target
- Alternative Names
- Low-density lipoprotein; LDL; Ldl cholesterol
- Function
- Ihibitor
Related Resources
Cushing Syndrome
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-615 | Recombinant Anti-LDL Antibody | ELISA, WB, FC, FuncS | IgG |
| MOB-0162MZ | Recombinant Mouse Anti-Human Low Density Lipoprotein Receptor-Related Protein (LRP) (515kDa) Antibody (clone 9H2) | WB, IHC-FoFr, FC | Mouse antibody |
| HPAB-1067WJ | Recombinant Human Anti-LDL Antibody (IEIA8) | ELISA | Human IgG |
| HPAB-1068WJ | Recombinant Human Anti-LDL Antibody (IEI-E3) | ELISA | Human IgG |
| HPAB-1069WJ | Recombinant Human Anti-LDL Antibody (KTT-D6) | ELISA | Human IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-615-F(E) | Recombinant Anti-LDL Antibody Fab Fragment | ELISA, WB, FuncS | Fab |
| MHH-615-F(E) | Recombinant Human Anti-LDL Antibody Fab Fragment | RIA, IF, FuncS | Fab |
| HPAB-1067WJ-F(E) | Recombinant Human Anti-LDL Antibody Fab Fragment (IEIA8) | ELISA | Human Fab |
| HPAB-1068WJ-F(E) | Recombinant Human Anti-LDL Antibody Fab Fragment (IEI-E3) | ELISA | Human Fab |
| FAMAB-0056JF-F(E) | Human Anti-LDL Recombinant Antibody (clone IK17); Fab Fragment | FACS, WB, IHC, ELISA, FuncS | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-615-S(P) | Recombinant Anti-LDL Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| MHH-615-S(P) | Recombinant Human Anti-LDL Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| FAMAB-0056JF-S(P) | Human Anti-LDL Recombinant Antibody (clone IK17); scFv Fragment | FACS, WB, IHC, ELISA, FuncS | Human scFv |
| FAMAB-0185JF-S(P) | Mouse Anti-LDL Recombinant Antibody (clone 1H11); scFv Fragment | ICC, WB, ELISA, FuncS | Mouse scFv |
| FAMAB-0186JF-S(P) | Mouse Anti-LDL Recombinant Antibody (clone 4E4); scFv Fragment | ICC, WB, ELISA, FuncS | Mouse scFv |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0322MZ | Chicken Anti-LDL Polyclonal IgY | Anti-LDL antibody produced in chicken is suitable for indirect ELISA and WBting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200 | Chicken antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4301 | Hi-Affi™ Recombinant Rabbit Anti-LDL Monoclonal Antibody (SI390DS) | FC | IgG |
| MOR-4302 | Hi-Affi™ Recombinant Rabbit Anti-LDL Monoclonal Antibody (SI391DS) | FC | IgG |
| MOR-4303 | Hi-Affi™ Recombinant Rabbit Anti-LDL Monoclonal Antibody (SI392DS) | FC | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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