Mouse Anti-LEPR Recombinant Antibody (VS3-CJ34)
CAT#: VS3-CJ34
This product is a mouse antibody that recognizes LEPR.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human choroid plexus
Immunohistochemical staining of human choroid plexus shows strong membranous positivity in the ependymal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/ih_additional_tissue/30899/975_429_400x400.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (K-562, RPTEC TERT1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000116678-LEPR
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Specifications
- Immunogen
- Recombinant fragment of human LEPR (N-terminus)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human LEPR
- Species Reactivity
- Human
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).
Target
- Alternative Names
- CD295; LEP-RD; OB-R; OBR; LEPR
- Gene ID
- 3953
- UniProt ID
- P48357
- Sequence Similarities
- Belongs to the type I cytokine receptor family. Type 2 subfamily.
- Cellular Localization
- Cell membrane, Membrane, Secreted
- Post Translation Modifications
- On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation,for interaction with SOCS3 and SOCS3 mediated inhibition of leptin signaling. Phosphorylation on Tyr-1141 is required for STAT3 binding/activation. Phosphorylation of Tyr-1079 has a more accessory role.
- Protein Refseq
- NP_001003679.1; NP_001003680.1; NP_001185616.1
- Function
- Receptor for hormone LEP/leptin (Probable) (PubMed:22405007).
On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones (By similarity) (PubMed:9537324).
In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity (PubMed:25060689, PubMed:12504075, PubMed:8805376).
Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis. Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus. Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T -ells. Leptin increases Th1 and suppresses Th2 cytokine production (By similarity).
Isoform A:
May transport LEP across the blood-brain barrier. Binds LEP and mediates LEP endocytosis. Does not induce phosphorylation of and activate STAT3.
Isoform E:
Antagonizes Isoform A and isoform B-mediated LEP binding and endocytosis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
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Secondary Antibody
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Recommended Dilution Buffer
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- Product Name
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| HPAB-0132-YC-S(P) | Human Anti-LEPR Recombinant Antibody; scFv Fragment (HPAB-0132-YC-S(P)) | ELISA, FC | Human scFv |
| HPAB-0133-YC-S(P) | Human Anti-LEPR Recombinant Antibody; scFv Fragment (HPAB-0133-YC-S(P)) | ELISA, FC | Human scFv |
| HPAB-0134-YC-S(P) | Human Anti-LEPR Recombinant Antibody; scFv Fragment (HPAB-0134-YC-S(P)) | ELISA, FC | Human scFv |
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| HPAB-N0167-YC-S(P) | Human Anti-LEPR Recombinant Antibody (HPAB-N0167-YC-S(P)); scFv Fragment | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3987 | Anti-LEPR Immunohistochemistry Kit | IHC | |
| VS-0525-XY3988 | Anti-Mouse LEPR Immunohistochemistry Kit | IHC |
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