Mouse Anti-LIN28A Recombinant Antibody (VS7-0425-WR300)
CAT#: VS7-0425-WR300
This product is a mouse IgG antibody that binds to human LIN28A. The antibody recognizes specific amino acid domain of LIN-28 (aa93-209). It can be used for WB, IF, ICC, and ELISA assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line AF22
Immunofluorescent staining of human cell line AF22 shows localization to nucleoli & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68814/1783_E7_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human testis
High expression. RNA expression: 5.2 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_A_6_6_selected.jpg
Normal Tissue
Figure 3 IHC staining of human endometrium
Low expression. RNA expression: 0.1 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_B_5_3_val_selected.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_A_9_5.jpg
Normal Tissue
Figure 6 Placenta
Trophoblastic cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_A_1_7.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (NTERA-2, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131914-LIN28A
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Specifications
- Immunogen
- Recombinant LIN-28 (aa93-209) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human LIN28A
- Species Reactivity
- Human
- Molecule Class
- Rna-Binding Protein
- Applications
- WB, IF, ICC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- protein G affinity chromatography
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A
- Gene ID
- 79727
- UniProt ID
- Q9H9Z2
- Long Name
- Lin-28 homolog A
- Cellular Localization
- Cytoplasm; Endoplasmic reticulum; Nucleus
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_078950.1
- Function
- RNA-binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "LIN28A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1638MZ | Recombinant Mouse Anti-Human LIN28A Antibody (clone 66DU69.23.2) | ELISA, IHC-P, WB | Mouse antibody |
| MRO-0925-CN | Recombinant Rabbit Anti-LIN28A Monoclonal Antibody (JA10-63) | WB, IP, FC | Rabbit IgG |
| VS3-QX690 | Mouse Anti-LIN28A Recombinant Antibody (clone A4-A6) | WB, ICC, IHC | Mouse IgG2b |
| VS3-QX691 | Mouse Anti-LIN28A Recombinant Antibody (clone 6D1F9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-WK220 | Rabbit Anti-LIN28A Recombinant Antibody (clone JA10-63) | WB, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2060 | Hi-Affi™ Recombinant Rabbit Anti-LIN28A Monoclonal Antibody (DS2060AB) | FC, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC662 | CytoStream™ Rabbit Anti-LIN28A Recombinant Antibody (VS13-YC662) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4037 | Anti-LIN28A Immunohistochemistry Kit | IHC | |
| VS-0525-XY4038 | Anti-Mouse LIN28A Immunohistochemistry Kit | IHC | |
| VS-0525-XY4039 | Anti-Rat LIN28A Immunohistochemistry Kit | IHC |
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