Mouse Anti-LIN28A Recombinant Antibody (VS7-0425-WR300) (CAT#: VS7-0425-WR300)
This product is a mouse IgG antibody that binds to human LIN28A. The antibody recognizes specific amino acid domain of LIN-28 (aa93-209). It can be used for WB, IF, ICC, and ELISA assays.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line AF22
(Immunofluorescent staining of human cell line AF22 shows localization to nucleoli & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68814/1783_E7_1_selected.jpg
(Immunofluorescent staining of human cell line AF22 shows localization to nucleoli & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68814/1783_E7_1_selected.jpg
Figure 2 IHC staining of human testis
(High expression. RNA expression: 5.2 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_A_6_6_selected.jpg
(High expression. RNA expression: 5.2 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_A_6_6_selected.jpg
Figure 3 IHC staining of human endometrium
(Low expression. RNA expression: 0.1 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_B_5_3_val_selected.jpg
(Low expression. RNA expression: 0.1 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_B_5_3_val_selected.jpg
Figure 4 Colon
(Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_A_8_3.jpg
(Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_A_8_3.jpg
Figure 5 Kidney
(Cells in tubules
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_A_9_5.jpg
(Cells in tubules
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_A_9_5.jpg
Figure 6 Placenta
(Trophoblastic cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_A_1_7.jpg
(Trophoblastic cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20785/164815_A_1_7.jpg
Figure 7 RNA cell line category: Group enriched (NTERA-2, SuSa)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131914-LIN28A
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131914-LIN28A
Specifications
- Immunogen
- Recombinant LIN-28 (aa93-209) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human LIN28A
- Species Reactivity
- Human
- Molecule Class
- Rna-Binding Protein
- Applications
- WB, IF, ICC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- protein G affinity chromatography
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A
- Gene ID
- 79727
- UniProt ID
- Q9H9Z2
- Long Name
- Lin-28 homolog A
- Cellular Localization
- Cytoplasm; Endoplasmic reticulum; Nucleus
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_078950.1
- Function
- RNA-binding
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1638MZ | Recombinant Mouse Anti-Human LIN28A Antibody (clone 66DU69.23.2) | ELISA, IHC-P, WB | Mouse antibody |
| MRO-0925-CN | Recombinant Rabbit Anti-LIN28A Monoclonal Antibody (JA10-63) | WB, IP, FC | Rabbit IgG |
| MRO-0926-CN | Recombinant Mouse Anti-LIN28A Monoclonal Antibody (A4-A6) | WB, IF, IHC | Mouse IgG2b |
| VS3-QX690 | Mouse Anti-LIN28A Recombinant Antibody (clone A4-A6) | WB, ICC, IHC | Mouse IgG2b |
| VS3-QX691 | Mouse Anti-LIN28A Recombinant Antibody (clone 6D1F9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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