Rabbit Anti-LMAN1 Recombinant Antibody (clone R01-8R1)
CAT#: VS3-XY3418
It is a recombinant rabbit antibody targeting the LMAN1 (Lectin, mannose binding 1), which may recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors. It recognizes and binds to human LMAN1. It also has cross-reactivity against mouse and rat LMAN1. It has been determined using Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line EFO-21
Immunofluorescent staining of human cell line EFO-21 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/2063_G6_7_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity with granular pattern in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2320/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human breast
Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_B_8_5.jpg
Normal Tissue
Figure 5 Thyroid gland
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_B_3_5.jpg
Normal Tissue
Figure 6 Colon
Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000074695-LMAN1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat LMAN1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-8R1
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Factor V And Factor Viii, Combined Deficiency of, 2
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- MR60; gp58; F5F8D; FMFD1; MCFD1; ERGIC53; ERGIC-53
- Gene ID
- 3998
- UniProt ID
- P49257
- Long Name
- Lectin, mannose binding 1
- Cellular Localization
- Golgi apparatus membrane, Endoplasmic reticulum membrane
- Post Translation Modifications
- The N-terminal may be partly blocked. (P49257-LMAN1_HUMAN)
Glycosylation at Thr276, Thr385, Ser425, Thr432, and Thr433 (NX_P49257 [NX_P49257-1])
Ubiquitination at Lys346 and Lys372 (NX_P49257 [NX_P49257-1])
Modification sites at PhosphoSitePlus (P49257)
Glycosylation from GlyGen (P49257) 9 sites, 5 O-linked glycans (9 sites)
- Protein Refseq
- NP_005561.1
- Function
- Mannose-specific lectin.
May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both.
The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "LMAN1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2385z | Mouse Anti-LMAN1 Recombinant Antibody (clone 15A2) | WB, FC, ICC, IF, IHC | Mouse IgG1 |
| VS3-FY849 | Recombinant Rabbit Anti-LMAN1 Antibody (clone R03-5C3) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0324MZ | Chicken Anti-LMAN1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2065 | Hi-Affi™ Recombinant Rabbit Anti-LMAN1 Monoclonal Antibody (DS2065AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY307 | Mouse Anti-NHP LMAN1 Recombinant Antibody (clone OTI1A8) | WB, IHC, IF, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1251 | Anti-LMAN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4049 | Anti-Mouse LMAN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4048 | Anti-Human LMAN1 Immunohistochemistry Kit | IHC |
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