Recombinant Rabbit Anti-LMAN1 Antibody (clone R03-5C3)
CAT#: VS3-FY849
This product is a recombinant rabbit antibody that recognizes LMAN1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The antigen is synthetic peptide of human LMAN1. The clone R03-5C3 is specific for human LMAN1 .
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line EFO-21
Immunofluorescent staining of human cell line EFO-21 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/2063_G6_7_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity with granular pattern in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2320/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human breast
Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_B_8_5.jpg
Normal Tissue
Figure 5 Thyroid gland
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_B_3_5.jpg
Normal Tissue
Figure 6 Colon
Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37163/85396_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000074695-LMAN1
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IHC-P
Figure 1. Recombinant Rabbit Anti-LMAN1 Antibody (clone R03-5C3) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human tonsil using LMAN1 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide of human LMAN1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human LMAN1
- Species Reactivity
- Human
- Clone
- R03-5C3
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 58 kDa; Observed MW: 58 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Target
- Alternative Names
- Lectin, Mannose Binding 1 2 3 5Endoplasmic Reticulum-Golgi Intermediate Compartment Protein 53 2 3ER-Golgi Intermediate Compartment 53 KDa Protein 3 4Intracellular Mannose-Specific Lectin MR60 3 4ERGIC53 3 4F5F8D 3 4Gp58 3 4Coagulation Factor V-Factor VIII Combined Deficiency 2
- Gene ID
- 3998
- UniProt ID
- P49257
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "LMAN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2385z | Mouse Anti-LMAN1 Recombinant Antibody (clone 15A2) | WB, FC, ICC, IF, IHC | Mouse IgG1 |
| VS3-XY3418 | Rabbit Anti-LMAN1 Recombinant Antibody (clone R01-8R1) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0324MZ | Chicken Anti-LMAN1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2065 | Hi-Affi™ Recombinant Rabbit Anti-LMAN1 Monoclonal Antibody (DS2065AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY307 | Mouse Anti-NHP LMAN1 Recombinant Antibody (clone OTI1A8) | WB, IHC, IF, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1251 | Anti-LMAN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4049 | Anti-Mouse LMAN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4048 | Anti-Human LMAN1 Immunohistochemistry Kit | IHC |
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