Mouse Anti-LMNB1 Recombinant Antibody (clone 2B11D5)
CAT#: VS7-0425-WR303
This product is a mouse IgG1 antibody that binds to human LMNB1. The antibody 2B11D5 recognizes specific amino acid domain of human LMNB1 (AA: 413-583). It can be used for WB, IHC, ICC, and FC assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nuclear membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5269/950_C1_1_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/79757/1894_G3_4_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human colon
Immunohistochemical staining of human colon shows nuclear membrane positivity in glandular and stroma cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong nuclear membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5269/155655_A_3_1_selected.jpg
Subcellular Location and Protein Expression
Figure 6 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/79954/171056_B_6_1_selected.jpg
Normal Tissue
Figure 7 Cerebral cortex
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_B_8_5.jpg
Normal Tissue
Figure 8 Kidney
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_A_7_4.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_A_5_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113368-LMNB1
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Specifications
- Immunogen
- Recombinant human LMNB1 (AA: 413-583) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human LMNB1
- Species Reactivity
- Human
- Clone
- 2B11D5
- Molecule Class
- Intermediate Filament Family
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Leukodystrophy, demyelinating, autosomal dominant, adult-onset (ADLD); Microcephaly 26, primary, autosomal dominant (MCPH26)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- LMNB1; LMN2; Lamin-B1; LMN; LMNB
- Gene ID
- 4001
- UniProt ID
- P20700
- Long Name
- Lamin B1
- Cellular Localization
- Intermediate filament; Nucleus
- Post Translation Modifications
- Acetylation; Disulfide bond; Glycoprotein; Isopeptide bond; Lipoprotein; Methylation; Phosphoprotein; Prenylation; Ubl conjugation
- Protein Refseq
- NP_001185486.1
- Function
- Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:28716252, PubMed:32910914).; Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:28716252, PubMed:32910914).; The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:28716252, PubMed:32910914).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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