This product is a mouse antibody that recognizes LMNB1.
Figure 1 Mouse Anti-LMNB1 Recombinant Antibody (clone 7C11) (MOB-0073F) in IF
Immunofluorescence analysis of Hela cells. 1. AMPKα1/2 (phosphoThr183/172) polyclonal antibody (green) is diluted at 1:200 (overnight at 4°C). (Red) Dilute at 1:200 (overnight at 4°C). 2. Goat anti-rabbit Alexa Fluor 488 is diluted 1:1000 (room temperature, 50 minutes). Goat anti-mouse Alexa Fluor 594 is diluted 1:1000 (room temperature, 50 minutes).
Figure 2 Mouse Anti-LMNB1 Recombinant Antibody (clone 7C11) (MOB-0073F) in IHC
Immunohistochemical analysis of paraffin-embedded human uterine tissue. 1. The antibody is diluted 1:200 (overnight at 4°C). 2. Sodium citrate pH 6.0 is used for antibody repair (> 98°C, 20 minutes). 3. Dilute the secondary antibody at 1:200 (room temperature, 30min). The negative control is only used by the secondary antibody.
Figure 3 Mouse Anti-LMNB1 Recombinant Antibody (clone 7C11) (MOB-0073F) in IF
Immunofluorescence analysis of human lung cancer tissue. 1. Lamin B1 Monoclonal Antibody (7C11) (red) 1:200 (4°C, overnight) dilution. 2. The Cy3 labeled secondary antibody was diluted 1:300 (room temperature, 50 minutes). 3. Picture B: DAPI (blue) for 10 minutes. Figure A: Goal. Picture B: DAPI. Picture C: The merger of A+B.
Figure 4 Mouse Anti-LMNB1 Recombinant Antibody (clone 7C11) (MOB-0073F) in WB
1) HepG2, 2) 293T, 3) Mouse brain tissue, 4) Western blot analysis of rat brain tissue, diluted 1:5000. The nucleus extracted by Minute TMCytoplasmic and Nuclear Fractionation kit.
Figure 5 Mouse Anti-LMNB1 Recombinant Antibody (clone 7C11) (MOB-0073F) in IP
1) Mouse brain tissue lysate 2) IP product: IP dilution 1:200.
Figure 6 Mouse Anti-LMNB1 Recombinant Antibody (clone 7C11) (MOB-0073F) in WB
Western blot analysis of lysates from 1) HepG2, 2) 293T, 3) mouse brain tissue cells, 4) rat brain tissue cells, (green) primary antibody diluted 1:1000, overnight at 4°C, secondary antibody (Dilution 1:10000), 37°C for 1 hour. (Red) Actin β polyclonal antibody diluted 1:5000 as loading control, overnight at 4°C, secondary antibody (diluted 1:10000, 37°C for 1 hour.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1818z | Mouse Anti-LMNB1 Recombinant Antibody (clone 19G6) | ELISA, IHC, WB | Mouse IgG2a, κ |
MOB-2469CT | Recombinant Mouse anti-Chicken LMNB1 Monoclonal antibody (T70) | WB | |
MRO-2006-CN | Rabbit Anti-LMNB1 Polyclonal Antibody (MRO-2006-CN) | WB | Rabbit IgG |
MOB-0074F | Mouse Anti-LMNB1 Recombinant Antibody (clone 7C11), AbFluor™ 350 | WB, IHC-P, IF, IP | Mouse IgG |
ZG-0264C | Mouse Anti-LMNB1 Recombinant Antibody (ZG-0264C) | WB | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2068 | Hi-Affi™ Recombinant Rabbit Anti-LMNB1 Monoclonal Antibody (DS2068AB) | WB, IHC, IP | IgG |
MOR-0075-FY | Rabbit Anti-LMNB1 Recombinant Antibody (clone AFY0046) | ICC, IHC-P, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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