Mouse Anti-LRP1 Recombinant Antibody (clone 1F6C6)
CAT#: VS7-0425-WR310
This product is a mouse IgG1 antibody that binds to human LRP1. The antibody 1F6C6 recognizes specific amino acid domain of human LRP1 (AA: 20-155). It can be used for ICC and FC assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4182/1150_F4_7_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows moderate membranous positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4182/13067_A_2_7_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18621/41781_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Fibroblasts
Staining: High
Intensity: Strong
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18621/41781_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18621/41781_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22903/51316_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18621/41781_A_4_6.jpg
Normal Tissue
Figure 8 Soft tissue
Fibroblasts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18621/41781_B_2_8.jpg
Normal Tissue
Figure 9 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18621/41781_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, fHDF/TERT166, HSkMC, hTERT-RPE1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000123384-LRP1
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Specifications
- Immunogen
- Recombinant human LRP1 (AA: 20-155) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human LRP1
- Species Reactivity
- Human
- Clone
- 1F6C6
- Molecule Class
- Low-Density Lipoprotein Receptor (LDLR)
- Applications
- ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Keratosis pilaris atrophicans (KPA); Developmental dysplasia of the hip 3 (DDH3)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- LRP1; low density lipoprotein receptor-related protein 1; APR; LRP; A2MR; CD91; APOER; LRP1A; TGFBR5; IGFBP3R; prolow-density lipoprotein receptor-related protein 1; LRP-1; type V tgf-beta receptor; apolipoprotein E receptor; alpha-2-macroglobulin recepto
- Gene ID
- 4035
- UniProt ID
- Q07954
- Long Name
- LDL receptor related protein 1
- Cellular Localization
- Cell membrane; Coated pit; Cytoplasm; Cytoskeleton; Golgi apparatus; Membrane; Nucleus
- Post Translation Modifications
- Acetylation; Disulfide bond; Glycoprotein; Phosphoprotein
- Protein Refseq
- NP_002323.2
- Function
- Developmental protein; Receptor
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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- CAT
- Product Name
Secondary Antibody
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Reconstitution Buffer
- CAT
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Recommended Dilution Buffer
- CAT
- Product Name
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