CytoStream™ Rabbit Anti-LTA4H Recombinant Antibody (VS13-YC675)
CAT#: VS13-YC675
The CytoStream™ Rabbit Anti-LTA4H Recombinant Antibody is specific for human, mouse and rat LTA4H. LTA4H encodes a hydrolase for leukotriene B4 biosynthesis, involved in pro-inflammatory mediation and degradation activities influencing COPD biomarkers. The anti-LTA4H antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8399/75_D10_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in cortical cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8399/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human fallopian tube
Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17017/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human colon
Immunohistochemical staining of human colon shows nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15221/37901_A_9_3_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17017/38426_B_7_5.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8399/23467_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8399/23467_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8399/23467_A_6_6.jpg
Normal Tissue
Figure 9 Bone marrow
Hematopoietic cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8399/23467_B_6_4.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15221/37901_A_9_8.jpg
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Specifications
- Immunogen
- Recombinant human LTA4H.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat LTA4H
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
- Related Disease
- chronic obstructive pulmonary disease (COPD)
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "LTA4H"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2098 | Hi-Affi™ Recombinant Rabbit Anti-LTA4H Monoclonal Antibody (DS2098AB) | FC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0129U | Rabbit Anti-LTA4H Recombinant Antibody (clone 6G7) | ELISA, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC583 | AbPlus™ Anti-LTA4H Magnetic Beads (VS-0724-YC583) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY310 | Mouse Anti-NHP LTA4H Recombinant Antibody (clone OTI1E9) | WB, IHC, IP | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4117 | Anti-LTA4H Immunohistochemistry Kit | IHC | |
| VS-0525-XY4118 | Anti-Mouse LTA4H Immunohistochemistry Kit | IHC | |
| VS-0525-XY4119 | Anti-Rat LTA4H Immunohistochemistry Kit | IHC |
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