Rabbit Anti-MAD2L2 Recombinant Antibody (clone 4D8) (CAT#: ZG-0450U)
Rabbit Anti-MAD2L2 Antibody (clone 4D8) displayed high specificity against the MAD2L2 protein of Human.
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WB
Figure 1 Rabbit Anti-MAD2L2 Antibody (ZG-0450U) in WB
Western Blot
Positive WB detected in: 293 whole cell lysate
All lanes: Mad2L2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
IHC
Figure 2 Rabbit Anti-MAD2L2 Antibody (ZG-0450U) in IHC
IHC image of ZG-0450U diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 3 Rabbit Anti-MAD2L2 Antibody (ZG-0450U) in IF
Immunofluorescence staining of Hela Cells with ZG-0450U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human Mad2L2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MAD2L2
- Species Reactivity
- Human
- Clone
- 4D8
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Fanconi Anemia, Complementation Group V and Fanconi Anemia, Complementation Group A.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- REV7; FANCV; MAD2B; POLZ2; mitotic spindle assembly checkpoint protein MAD2B; MAD2 (mitotic arrest deficient, yeast, homolog)-like 2; MAD2 mitotic arrest deficient-like 2; MAD2-like protein 2; REV7 homolog; hREV7; mitotic arrest deficient 2-like protein 2; mitotic arrest deficient homolog-like 2; polymerase (DNA-directed), zeta 2, accessory subunit
- Gene ID
- 10459
- UniProt ID
- Q9UI95
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| MOR-2117 | Hi-Affi™ Recombinant Rabbit Anti-MAD2L2 Monoclonal Antibody (DS2117AB) | IP, ICC/IF, FC, IHC-P, WB | IgG |
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