Rabbit Anti-MADD Recombinant Antibody (clone R03-8F0)
CAT#: VS3-FY1995
Clone R03-8F0 is a rabbit recombinant monoclonal antibody that has been genetically engineered to specifically target human MADD. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. This DENN antibody was verified by western blot analysis of DENN in Hela, A549, HL-60 lysates and immunohistochemistry analysis of paraffin-embedded human breast cancer.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38568/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38568/91813_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38568/91813_A_8_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38568/91813_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38568/91813_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38568/91813_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000110514-MADD
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WB
Figure 1 Anti-MADD Antibody (VS3-FY1995) in WB.
The DENN expression in Hela, A549, HL-60 lysates were detected using the anti-DENN by western blot assay.
IHC
Figure 2 Anti-MADD Antibody (VS3-FY1995) in IHC.
Immunohistochemistry staining of paraffin-embedded human breast cancer using the anti-DENN antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide of human DENN
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MADD
- Species Reactivity
- Human
- Clone
- R03-8F0
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 183 kDa, while the observed molecular weight stands at 220 kDa.
- Related Disease
- Human breast cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- MAP Kinase Activating Death Domain; Differentially Expressed In Normal And Neoplastic Cells; Insulinoma Glucagonoma Clone 20; Rab3 GDP/GTP Exchange Factor; DENN; IG20
- Gene ID
- 8567
- UniProt ID
- Q8WXG6
- Sequence Similarities
- Belongs to the MADD family.
- Cellular Localization
- Cell membrane, Cell projection, Cytoplasm, Membrane
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q8WXG6)
Modification sites at neXtProt (NX_Q8WXG6)
Glycosylation from GlyGen (Q8WXG6) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- NP_001129415.1; NP_001129416.1; NP_001363500.1; NP_001363501.1; NP_001363502.1
- Function
- Plays a role in synaptic vesicle formation and in vesicle trafficking at the neuromuscular junction (By similarity).
Involved in up-regulating a post-docking step of synaptic exocytosis in central synapses (By similarity).
Probably by binding to the motor proteins KIF1B and KIF1A, mediates motor-dependent transport of GTP-RAB3A-positive vesicles to the presynaptic nerve terminals (By similarity).
Plays a role in TNFA-mediated activation of the MAPK pathway, including ERK1/2.
May link TNFRSF1A with MAP kinase activation.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MADD"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2119 | Hi-Affi™ Recombinant Rabbit Anti-MADD Monoclonal Antibody (DS2119AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4163 | Anti-MADD Immunohistochemistry Kit | IHC |
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