Rabbit Anti-MADD Recombinant Antibody (clone R03-8F0) (CAT#: VS3-FY1995)
Clone R03-8F0 is a rabbit recombinant monoclonal antibody that has been genetically engineered to specifically target human MADD. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. This DENN antibody was verified by western blot analysis of DENN in Hela, A549, HL-60 lysates and immunohistochemistry analysis of paraffin-embedded human breast cancer.
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WB
Figure 1 Anti-MADD Antibody (VS3-FY1995) in WB.
The DENN expression in Hela, A549, HL-60 lysates were detected using the anti-DENN by western blot assay.
IHC
Figure 2 Anti-MADD Antibody (VS3-FY1995) in IHC.
Immunohistochemistry staining of paraffin-embedded human breast cancer using the anti-DENN antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide of human DENN
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MADD
- Species Reactivity
- Human
- Clone
- R03-8F0
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 183 kDa, while the observed molecular weight stands at 220 kDa.
- Related Disease
- Human breast cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- MAP Kinase Activating Death Domain; Differentially Expressed In Normal And Neoplastic Cells; Insulinoma Glucagonoma Clone 20; Rab3 GDP/GTP Exchange Factor; DENN; IG20
- Gene ID
- 8567
- UniProt ID
- Q8WXG6
- Sequence Similarities
- Belongs to the MADD family.
- Cellular Localization
- Cell membrane, Cell projection, Cytoplasm, Membrane
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q8WXG6)
Modification sites at neXtProt (NX_Q8WXG6)
Glycosylation from GlyGen (Q8WXG6) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- NP_001129415.1; NP_001129416.1; NP_001363500.1; NP_001363501.1; NP_001363502.1
- Function
- Plays a role in synaptic vesicle formation and in vesicle trafficking at the neuromuscular junction (By similarity).
Involved in up-regulating a post-docking step of synaptic exocytosis in central synapses (By similarity).
Probably by binding to the motor proteins KIF1B and KIF1A, mediates motor-dependent transport of GTP-RAB3A-positive vesicles to the presynaptic nerve terminals (By similarity).
Plays a role in TNFA-mediated activation of the MAPK pathway, including ERK1/2.
May link TNFRSF1A with MAP kinase activation.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2119 | Hi-Affi™ Recombinant Rabbit Anti-MADD Monoclonal Antibody (DS2119AB) | WB, IHC, ICC | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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