Mouse Anti-MAP2 Recombinant Antibody (VS3-CJ67) (CAT#: VS3-CJ67)
This product is a mouse antibody that recognizes MAP2.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line AF22
(Immunofluorescent staining of human cell line AF22 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/1895_G7_3_selected.jpg
(Immunofluorescent staining of human cell line AF22 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/1895_G7_3_selected.jpg
Figure 2 IF staining of human cell line SH-SY5Y
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12558/1927_F10_1_selected.jpg
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12558/1927_F10_1_selected.jpg
Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/24187_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/24187_A_7_3.jpg
Figure 4 Cerebral cortex
(Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/24187_B_7_5.jpg
(Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/24187_B_7_5.jpg
Figure 5 RNA cell line category: Cell line enhanced (AF22, NTERA-2, SCLC-21H, SH-SY5Y)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000078018-MAP2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000078018-MAP2
Specifications
- Immunogen
- Recombinant fragment of human MAP2 (C-terminus)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MAP2
- Species Reactivity
- Human
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).
Target
- Alternative Names
- MAP-2A; MAP2B; MAP2C; MAP2
- Gene ID
- 4133
- UniProt ID
- P11137
- Cellular Localization
- Cell projection, Cytoplasm, Cytoskeleton, Microtubule
- Post Translation Modifications
- Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity).
Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity).
- Protein Refseq
- NP_001034627.1; NP_002365.3; NP_114033.2
- Function
- The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1671z | Mouse Anti-MAP2 Recombinant Antibody (clone 22D3) | WB, ELISA, ICC, IHC, IP | Mouse IgG1, κ |
| MOB-0095F | Mouse Anti-MAP2 Recombinant Antibody (clone 7D4) | IHC-P, IF | Mouse IgG |
| ZG-0267C | Mouse Anti-MAP2 Recombinant Antibody (ZG-0267C) | WB | Mouse IgG |
| VS3-WK90 | Mouse Anti-MAP2 Recombinant Antibody (clone F7-B7) | WB, ELISA | Mouse IgG1 |
| VS3-XY1024 | Mouse Anti-MAP2 Recombinant Antibody (clone 5B7) | ELISA, WB, FC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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