Mouse Anti-MAP2 Recombinant Antibody (clone 7D4)
CAT#: MOB-0095F
This product is a mouse antibody that recognizes MAP2.
Tested Data
Gene Expression
IF
Figure 1 Mouse Anti-MAP2 Recombinant Antibody (clone 7D4) (MOB-0095F) in IF
Immunofluorescence analysis of Hela cells. 1. AQP2 polyclonal antibody (green) is diluted at 1:200 (overnight at 4°C). (Red) Dilute at 1:200 (overnight at 4°C). 2. Goat anti-rabbit Alexa Fluor 488 diluted 1:1000 (room temperature, 50 minutes). Goat Anti Mouse Alexa Fluor594 diluted 1:1000 (room temperature, 50 minutes).
IHC
Figure 2 Mouse Anti-MAP2 Recombinant Antibody (clone 7D4) (MOB-0095F) in IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue. 1. MAP2 monoclonal antibody (7D4) was diluted 1:200 (4°C, overnight). 2. Sodium citrate pH 6.0 is used for antibody repair (>98°C, 20 minutes). 3. The secondary antibody is diluted 1:200 (room temperature, 30 minutes). The negative control is only used by the secondary antibody.
IHC
Figure 3 Mouse Anti-MAP2 Recombinant Antibody (clone 7D4) (MOB-0095F) in IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1. MAP2 monoclonal antibody (7D4) was diluted at 1:200 (4°C, overnight). 2. Sodium citrate pH 6.0 is used for antibody repair (>98°C, 20 minutes). 3. The secondary antibody is diluted 1:200 (room temperature, 30 minutes). The negative control is only used by the secondary antibody.
IF
Figure 4 Mouse Anti-MAP2 Recombinant Antibody (clone 7D4) (MOB-0095F) in IF
Immunofluorescence analysis of mouse brain tissue. 1. MAP2 Monoclonal Antibody (7D4) (red) is diluted at 1:200 (4°C, overnight). 2. The Cy3 labeled secondary antibody was diluted 1:300 (room temperature, 50 minutes). 3. Picture B: DAPI (blue) for 10 minutes. Figure A: Goal. Figure B: DAPI. Figure C: The merger of A+B.
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Subcellular Location
Figure 1 IF staining of human cell line AF22
Immunofluorescent staining of human cell line AF22 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/1895_G7_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12558/1927_F10_1_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/24187_A_7_3.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/24187_B_7_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (AF22, NTERA-2, SCLC-21H, SH-SY5Y)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000078018-MAP2
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MAP2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 7D4
- Applications
- IHC-P, IF
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunohistologyp-Paraffin Sections, Immunofluorescence.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "MAP2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0797MZ | Chicken Anti-MAP2 Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| BRD-1159MZ | Chicken Anti-Microtubule Associated Protein 2 (MAP2) Polyclonal IgY | ICC | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2130 | Hi-Affi™ Recombinant Rabbit Anti-MAP2 Monoclonal Antibody (DS2130AB) | IHC-P, IP, WB | IgG |
| MOR-0097-FY | Rabbit Anti-MAP2 Recombinant Antibody (clone AFY0068) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX713 | Mouse Anti-MAP2 Recombinant Antibody (clone 1D7-B9-C8-F6) | WB, IP, ICC | Mouse IgG2b |
| VS3-CJ67 | Mouse Anti-MAP2 Recombinant Antibody (VS3-CJ67) | WB, ELISA | Mouse IgG |
| VS4-CJ25 | Recombinant Mouse Anti-MAP2 Antibody (VS4-CJ25) | ELISA, IHC | Mouse IgG |
| VS4-CJ26 | Recombinant Mouse Anti-MAP2 Antibody (VS4-CJ26) | ELISA, WB, ICC | Mouse IgG2b |
| VS7-0425-WR331 | Mouse Anti-MAP2 Recombinant Antibody (VS7-0425-WR331) | WB, IF, ICC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY161 | CytoStream™ Mouse Anti-MAP2 Recombinant Antibody (VS-0225-XY161) | FC | Mouse IgG1, kappa |
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