Mouse Anti-MAP2 Recombinant Antibody (clone 7D4)
CAT#: MOB-0095F
This product is a mouse antibody that recognizes MAP2.
Tested Data
Gene Expression
IF
Figure 1 Mouse Anti-MAP2 Recombinant Antibody (clone 7D4) (MOB-0095F) in IF
Immunofluorescence analysis of Hela cells. 1. AQP2 polyclonal antibody (green) is diluted at 1:200 (overnight at 4°C). (Red) Dilute at 1:200 (overnight at 4°C). 2. Goat anti-rabbit Alexa Fluor 488 diluted 1:1000 (room temperature, 50 minutes). Goat Anti Mouse Alexa Fluor594 diluted 1:1000 (room temperature, 50 minutes).
IHC
Figure 2 Mouse Anti-MAP2 Recombinant Antibody (clone 7D4) (MOB-0095F) in IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue. 1. MAP2 monoclonal antibody (7D4) was diluted 1:200 (4°C, overnight). 2. Sodium citrate pH 6.0 is used for antibody repair (>98°C, 20 minutes). 3. The secondary antibody is diluted 1:200 (room temperature, 30 minutes). The negative control is only used by the secondary antibody.
IHC
Figure 3 Mouse Anti-MAP2 Recombinant Antibody (clone 7D4) (MOB-0095F) in IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1. MAP2 monoclonal antibody (7D4) was diluted at 1:200 (4°C, overnight). 2. Sodium citrate pH 6.0 is used for antibody repair (>98°C, 20 minutes). 3. The secondary antibody is diluted 1:200 (room temperature, 30 minutes). The negative control is only used by the secondary antibody.
IF
Figure 4 Mouse Anti-MAP2 Recombinant Antibody (clone 7D4) (MOB-0095F) in IF
Immunofluorescence analysis of mouse brain tissue. 1. MAP2 Monoclonal Antibody (7D4) (red) is diluted at 1:200 (4°C, overnight). 2. The Cy3 labeled secondary antibody was diluted 1:300 (room temperature, 50 minutes). 3. Picture B: DAPI (blue) for 10 minutes. Figure A: Goal. Figure B: DAPI. Figure C: The merger of A+B.
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Subcellular Location
Figure 1 IF staining of human cell line AF22
Immunofluorescent staining of human cell line AF22 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/1895_G7_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12558/1927_F10_1_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/24187_A_7_3.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/24187_B_7_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (AF22, NTERA-2, SCLC-21H, SH-SY5Y)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000078018-MAP2
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Antibody Isotype
- IgG
- Specificity
- Human MAP2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 7D4
- Applications
- IHC-P, IF
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunohistologyp-Paraffin Sections, Immunofluorescence.
Target
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Excellent Dendritic Staining
Clone 7D4 is fantastic for visualizing dendritic shape in neurons. We used it in Immunofluorescence assays on rat and mouse tissue, and the staining of microtubule-associated protein 2 was very specific. It effectively highlights the cytoskeleton in neurogenesis studies.
Works In Paraffin Sections
This mouse recombinant antibody performed well in Immunohistochemistry on paraffin sections (IHC-P). It showed strong reactivity with Human MAP2. The antibody is stable at 4°C for short periods, which is convenient during our staining weeks.
Q&As
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What species does this MAP2 antibody cross-react with?
A: This Mouse Anti-MAP2 Recombinant Antibody (clone 7D4) recognizes MAP2 from Human, Mouse, and Rat samples.
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Is this antibody suitable for paraffin sections?
A: Yes, this antibody has been tested for use in Immunohistology-Paraffin Sections (IHC-P) as well as Immunofluorescence (IF).
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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