Rabbit Anti-MAP2K1 (pS298) Recombinant Antibody (clone R07-1I6)
CAT#: VS3-WK2003
This product is a rabbit recombinant antibody that is specific to phosphorylated MAP2K1. The target of this antibody is phospho-MAP2K1 (Ser298). The clone R07-1I6 can be used in WB analysis at a dilution of 1/500-1/1000 and IF analysis at a dilution of 1/50-1/200. The molecular weight of MAP2K1 (pSer298) observed in WB experiments using this antibody is 43 kDa.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3834/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3834/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/57722_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/57722_A_8_3.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/57722_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169032-MAP2K1
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WB
Figure 1 Rabbit Anti-MAP2K1 (pSer298) Recombinant Antibody (clone R07-1I6) in WB.
Western blot analysis of phospho-MAP2K1 (Ser298) in C6, 3T3, Hela lysates using anti-MAP2K1 (pSer298) antibody, Cat# VS3-WK2003.
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues Ser298 of human MEK1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser298 of human, mouse and rat MAP2K1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-1I6
- Applications
- WB, ICC, IF
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "MAP2K1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0340MZ | Chicken Anti-MAP2K1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2131 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K1 Monoclonal Antibody (DS2131AB) | ICC, IHC-P, IP, WB | IgG |
| MOR-4693 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K1 Monoclonal Antibody (TH207DS) | WB, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2344-CN | Recombinant Rabbit Anti-MAP2K1 (phosphorylated Ser298) Monoclonal Antibody (CBACN-635) | WB, IF, IHC | Rabbit IgG |
| ZG-0277C | Mouse Anti-MAP2K1 Recombinant Antibody (ZG-0277C) | WB | Mouse IgG |
| VS3-WK852 | Mouse Anti-MAP2K1 Recombinant Antibody (VS3-WK852) | WB, FC | Mouse IgG |
| VS3-WK855 | Rabbit Anti-MAP2K1 Recombinant Antibody (VS3-WK855) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-FY877 | Recombinant Rabbit Anti-MAP2K1 Antibody (clone R01-3C2) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY180 | AbPlus™ Anti-MAP2K1 Magnetic Beads (CBACN-357) | IP, Protein Purification | |
| VS-0724-YC941 | AbPlus™ Anti-MAP2K1 Magnetic Beads (VS-0724-YC941) | IP, Protein Purification | |
| VS-0724-YC968 | AbPlus™ Anti-Map2k1 Magnetic Beads (VS-0724-YC968) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4205 | Anti-MAP2K1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4206 | Anti-Mouse MAP2K1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4207 | Anti-Rat MAP2K1 Immunohistochemistry Kit | IHC |
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