Rabbit Anti-MAP2K1 Recombinant Antibody (clone 3F10) (CAT#: ZG-0452U)
Rabbit Anti-MAP2K1 Antibody (clone 3F10) displayed high specificity against the MAP2K1 protein of Human.
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WB
Figure 1 Rabbit Anti-MAP2K1 Antibody (ZG-0452U) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate
All lanes: MAP2K1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44, 41 kDa
Observed band size: 44 kDa
IHC
Figure 2 Rabbit Anti-MAP2K1 Antibody (ZG-0452U) in IHC
IHC image of ZG-0452U diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IP
Figure 3 Rabbit Anti-MAP2K1 Antibody (ZG-0452U) in IP
Immunoprecipitating MAP2K1 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0452U in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0452U(2µg)+ Hela whole cell lysate(500µg)
Lane 3: Hela whole cell lysate (10µg)
Specifications
- Immunogen
- A synthesized peptide derived from human MEK1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MAP2K1
- Species Reactivity
- Human
- Clone
- 3F10
- Applications
- ELISA, WB, IHC, IP
- Conjugate
- Unconjugated
- Related Disease
- Cardiofaciocutaneous Syndrome 3 and Melorheostosis, Isolated.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000
Target
- Alternative Names
- MEL; CFC3; MEK1; MKK1; MAPKK1; PRKMK1; dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1; MAPKK 1; MEK 1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1); EC 2.7.12.2p
- Gene ID
- 5604
- UniProt ID
- Q02750
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0340MZ | Chicken Anti-MAP2K1 Polyclonal IgY | WB | Chicken antibody |
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For Research Use Only. Not For Clinical Use.
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