Rabbit Anti-MAP2K1 Recombinant Antibody (clone 9B6) (CAT#: ZG-0453U)
Rabbit Anti-MAP2K1 Antibody (clone 9B6) displayed high specificity against the MAP2K1 protein of Human and Rat.
Specific Inquiry
WB
Figure 1 Rabbit Anti-MAP2K1 Antibody (ZG-0453U) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, A549 whole cell lysate, U251 whole cell lysate, Rat brain tissue
All lanes: MAP2K1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44, 41 kDa
Observed band size: 44 kDa
IHC
Figure 2 Rabbit Anti-MAP2K1 Antibody (ZG-0453U) in IHC
IHC image of ZG-0453U diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IP
Figure 3 Rabbit Anti-MAP2K1 Antibody (ZG-0453U) in IP
Immunoprecipitating MAP2K1 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0453U in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0453U(2µg)+ Hela whole cell lysate(500µg)
Lane 3: Hela whole cell lysate (10µg)
Specifications
- Immunogen
- A synthesized peptide derived from human MEK1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MAP2K1
- Species Reactivity
- Human, Rat
- Clone
- 9B6
- Applications
- ELISA, WB, IHC, IP
- Conjugate
- Unconjugated
- Related Disease
- Cardiofaciocutaneous Syndrome 3 and Melorheostosis, Isolated.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000
Target
- Alternative Names
- MEL; CFC3; MEK1; MKK1; MAPKK1; PRKMK1; dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1; MAPKK 1; MEK 1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1); EC 2.7.12.2
- Gene ID
- 5604
- UniProt ID
- Q02750
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1129CT | Recombinant Mouse anti-Human MAP2K1 Monoclonal antibody (8G2) | IP, WB | |
| MRO-2344-CN | Recombinant Rabbit Anti-MAP2K1 (phosphorylated Ser298) Monoclonal Antibody (CBACN-635) | WB, IF, IHC | Rabbit IgG |
| MRO-2345-CN | Recombinant Rabbit Anti-MAP2K1 (phosphorylated Thr292) Monoclonal Antibody (CBACN-636) | WB | Rabbit IgG |
| MRO-2346-CN | Recombinant Rabbit Anti-MAP2K1 (phosphorylated Ser218/Ser222) Monoclonal Antibody (CBACN-637) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-WK2003 | Rabbit Anti-MAP2K1 (pS298) Recombinant Antibody (clone R07-1I6) | WB, ICC, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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