Rabbit Anti-MAP2K1 Recombinant Antibody (VS3-FY2793)
CAT#: VS3-FY2793
This anti-MAP2K1 antibody is a rabbit recombinant monoclonal antibody that has been designed to bind the human MAP2K1 protein. It cross-reacts with mouse, rat species. The monoclonal antibody can be used in the area of Cardiofaciocutaneous Syndrome 3 and Melorheostosis, Isolated. The antibody can be utilized for detection of target protein by Immunoprecipitation (1/20), Western Blot (1/500-1/1000), Immunohistochemistry (1/50-1/100) and Immunocytochemistry (1/50-1/200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3834/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3834/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/57722_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/57722_A_8_3.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/57722_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169032-MAP2K1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MAP2K1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
- Conjugate
- Unconjugated, FITC
- Related Disease
- Cardiofaciocutaneous Syndrome 3 and Melorheostosis, Isolated
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunoprecipitation: 1/20
Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunocytochemistry: 1/50-1/200
Target
- Alternative Names
- Mitogen-Activated Protein Kinase Kinase 1; ERK Activator Kinase 1; MAPK/ERK Kinase 1; EC 2.7.12.2; MAPKK 1; PRKMK1; MEK 1; MEK1
- Gene ID
- 5604
- UniProt ID
- Q02750
- Sequence Similarities
- Belongs to the protein kinase superfamily.
- Cellular Localization
- Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body
Cytoplasm
Nucleus
Membrane
Peripheral membrane protein
- Post Translation Modifications
- Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (BRAF or MEKK1) positively regulates kinase activity (PubMed:29433126, 8131746).
Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK (PubMed:16129686).
MAPK1/ERK2 phosphorylation of Thr-292 oCcurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK
- Protein Refseq
- XP_016877900.1; XP_054234418.1; XP_054234419.1; NP_001397994.1
- Function
- Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway.
Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MAP2K1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0340MZ | Chicken Anti-MAP2K1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2131 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K1 Monoclonal Antibody (DS2131AB) | ICC, IHC-P, IP, WB | IgG |
| MOR-4693 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K1 Monoclonal Antibody (TH207DS) | WB, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2344-CN | Recombinant Rabbit Anti-MAP2K1 (phosphorylated Ser298) Monoclonal Antibody (CBACN-635) | WB, IF, IHC | Rabbit IgG |
| MRO-2345-CN | Recombinant Rabbit Anti-MAP2K1 (phosphorylated Thr292) Monoclonal Antibody (CBACN-636) | WB | Rabbit IgG |
| MRO-2346-CN | Recombinant Rabbit Anti-MAP2K1 (phosphorylated Ser218/Ser222) Monoclonal Antibody (CBACN-637) | WB, IF, IHC, IP | Rabbit IgG |
| ZG-0451U | Rabbit Anti-Phospho-MAP2K1 (T292) Recombinant Antibody (clone 4E7) | ELISA, WB | Rabbit IgG |
| ZG-0452U | Rabbit Anti-MAP2K1 Recombinant Antibody (clone 3F10) | ELISA, WB, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY180 | AbPlus™ Anti-MAP2K1 Magnetic Beads (CBACN-357) | IP, Protein Purification | |
| VS-0724-YC941 | AbPlus™ Anti-MAP2K1 Magnetic Beads (VS-0724-YC941) | IP, Protein Purification | |
| VS-0724-YC968 | AbPlus™ Anti-Map2k1 Magnetic Beads (VS-0724-YC968) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4205 | Anti-MAP2K1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4206 | Anti-Mouse MAP2K1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4207 | Anti-Rat MAP2K1 Immunohistochemistry Kit | IHC |
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