Rabbit Anti-MAP2K1 Recombinant Antibody (VS3-FY2793) (CAT#: VS3-FY2793)
This anti-MAP2K1 antibody is a rabbit recombinant monoclonal antibody that has been designed to bind the human MAP2K1 protein. It cross-reacts with mouse, rat species. The monoclonal antibody can be used in the area of Cardiofaciocutaneous Syndrome 3 and Melorheostosis, Isolated. The antibody can be utilized for detection of target protein by Immunoprecipitation (1/20), Western Blot (1/500-1/1000), Immunohistochemistry (1/50-1/100) and Immunocytochemistry (1/50-1/200).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3834/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3834/if_selected.jpg
Figure 3 IHC staining of human hippocampus
(Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/ihc_selected.jpg
(Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/ihc_selected.jpg
Figure 4 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3834/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3834/ihc_selected.jpg
Figure 5 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/57722_B_8_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/57722_B_8_5.jpg
Figure 6 Colon
(Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/57722_A_8_3.jpg
(Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/57722_A_8_3.jpg
Figure 7 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/57722_A_9_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/57722_A_9_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169032-MAP2K1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169032-MAP2K1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MAP2K1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
- Conjugate
- Unconjugated, FITC
- Related Disease
- Cardiofaciocutaneous Syndrome 3 and Melorheostosis, Isolated
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunoprecipitation: 1/20
Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunocytochemistry: 1/50-1/200
Target
- Alternative Names
- Mitogen-Activated Protein Kinase Kinase 1; ERK Activator Kinase 1; MAPK/ERK Kinase 1; EC 2.7.12.2; MAPKK 1; PRKMK1; MEK 1; MEK1
- Gene ID
- 5604
- UniProt ID
- Q02750
- Sequence Similarities
- Belongs to the protein kinase superfamily.
- Cellular Localization
- Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body
Cytoplasm
Nucleus
Membrane
Peripheral membrane protein
- Post Translation Modifications
- Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (BRAF or MEKK1) positively regulates kinase activity (PubMed:29433126, 8131746).
Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK (PubMed:16129686).
MAPK1/ERK2 phosphorylation of Thr-292 oCcurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK
- Protein Refseq
- XP_016877900.1; XP_054234418.1; XP_054234419.1; NP_001397994.1
- Function
- Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway.
Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1129CT | Recombinant Mouse anti-Human MAP2K1 Monoclonal antibody (8G2) | IP, WB | |
| MRO-0978-CN | Recombinant Rabbit Anti-MAP2K1 Monoclonal Antibody (CBACN-357) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-2344-CN | Recombinant Rabbit Anti-MAP2K1 (phosphorylated Ser298) Monoclonal Antibody (CBACN-635) | WB, IF, IHC | Rabbit IgG |
| ZG-0451U | Rabbit Anti-Phospho-MAP2K1 (T292) Recombinant Antibody (clone 4E7) | ELISA, WB | Rabbit IgG |
| ZG-0452U | Rabbit Anti-MAP2K1 Recombinant Antibody (clone 3F10) | ELISA, WB, IHC, IP | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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