Rabbit Anti-MAPKAPK2 Recombinant Antibody (VS13-YC703) (CAT#: VS13-YC703)
The Rabbit Anti-MAPKAPK2 Recombinant Antibody is specific for human and mouse MAPKAPK2. MAPKAPK2 encodes a Ser/Thr protein kinase regulated by p38 MAP kinase, involved in stress responses, nuclear export, and gene expression. Heat shock protein HSP27 is an in vivo substrate, with two different isoforms encoded by this gene. The anti-MAPKAPK2 antibody can be used for Western blot (1/500-1/1000), Immunoprecipitation (1/10-1/50).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45556/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45556/if_selected.jpg
Figure 2 IHC staining of human gall bladder
(Immunohistochemical staining of human gall bladder shows distinct cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10297/ihc_selected.jpg
(Immunohistochemical staining of human gall bladder shows distinct cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10297/ihc_selected.jpg
Figure 3 Breast
(Adipocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63708/145387_B_2_4.jpg
(Adipocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63708/145387_B_2_4.jpg
Figure 4 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63708/145387_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63708/145387_A_8_5.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63708/145387_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63708/145387_A_6_6.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162889-MAPKAPK2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162889-MAPKAPK2
Specifications
- Immunogen
- Recombinant human MAPKAP Kinase 2, aa 351-400.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse MAPKAPK2
- Species Reactivity
- Human, Mouse
- Applications
- WB, IP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunoprecipitation (IP): 1/10-1/50
Target
- Alternative Names
- Mitogen-Activated Protein Kinase-Activated Protein Kinase 2; MAPK-Activated Protein Kinase 2; MAPKAP Kinase 2; EC 2.7.11.1; MAPKAP-K2; MK-2;
- Gene ID
- 9261
- UniProt ID
- P49137
- Long Name
- Mitogen-Activated Protein Kinase-Activated Protein Kinase 2
- Cellular Localization
- Nucleus; Cytoplasm
- Function
- Signal transduction
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2161 | Hi-Affi™ Recombinant Rabbit Anti-MAPKAPK2 Monoclonal Antibody (DS2161AB) | ICC/IF, IHC-P, WB | IgG |
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