Rabbit Anti-MAPKAPK2 Recombinant Antibody (clone 4H8) (CAT#: ZG-0034U)

The purified Rabbit Anti-MAPKAPK2 Recombinant Antibody (clone 4H8) displayed high specificity against the MAPKAPK2 protein of human.


Specific Inquiry
  • Size:
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  • Purity:
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  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 Rabbit Anti-MAPKAPK2 Antibody (ZG-0034U) in WB

Figure 1 Rabbit Anti-MAPKAPK2 Antibody (ZG-0034U) in WB

Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, THP-1 whole cell lysate. All lanes: MAPKAPK2 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46, 43 kDa
Observed band size: 49 kDa

IHC

Figure 2 Rabbit Anti-MAPKAPK2 Antibody (ZG-0034U) in IHC

Figure 2 Rabbit Anti-MAPKAPK2 Antibody (ZG-0034U) in IHC

IHC image of ZG-0034U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.


Specifications

  • Immunogen
  • A synthesized peptide derived from human MAPKAP Kinase 2
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human MAPKAPK2
  • Species Reactivity
  • Human
  • Clone
  • 4H8
  • Applications
  • ELISA, WB, IHC
  • Conjugate
  • Unconjugated
  • Related Disease
  • Monocytic Leukemia and Tuberous Sclerosis 2

Product Property

  • Purification
  • Affinity purified
  • Concentration
  • See the COA
  • Buffer
  • PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
    WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200

Target

  • Alternative Names
  • MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2; EC 2.7.11.1; MAPKAPK2

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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