Rabbit Anti-MAPKAPK2 Recombinant Antibody (clone 4H8) (CAT#: ZG-0034U)
The purified Rabbit Anti-MAPKAPK2 Recombinant Antibody (clone 4H8) displayed high specificity against the MAPKAPK2 protein of human.
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WB
Figure 1 Rabbit Anti-MAPKAPK2 Antibody (ZG-0034U) in WB
Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, THP-1 whole cell lysate. All lanes: MAPKAPK2 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46, 43 kDa
Observed band size: 49 kDa
IHC
Figure 2 Rabbit Anti-MAPKAPK2 Antibody (ZG-0034U) in IHC
IHC image of ZG-0034U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human MAPKAP Kinase 2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MAPKAPK2
- Species Reactivity
- Human
- Clone
- 4H8
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Monocytic Leukemia and Tuberous Sclerosis 2
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2; EC 2.7.11.1; MAPKAPK2
- Gene ID
- 9261
- UniProt ID
- P49137
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2161 | Hi-Affi™ Recombinant Rabbit Anti-MAPKAPK2 Monoclonal Antibody (DS2161AB) | ICC/IF, IHC-P, WB | IgG |
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For Research Use Only. Not For Clinical Use.
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