Anti-MCL1 Antibody Prodrug, Protease Activated (01JF43)
CAT#: VS-1025-YC75
The Anti-MCL1 Antibody Prodrug, Protease Activated (01JF43) is an antibody prodrug comprised of a recombinant human antibody targeting mouse MCL1, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to MCL1 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to MCL1 effectively. MCL1 (Myeloid Cell Leukemia 1) is a key anti-apoptotic protein belonging to the BCL-2 family, highly expressed in many human tumors and critical for their survival. While the user specified "Mouse," its function is homologous to human MCL1, which is a powerful pro-survival oncogene. Its function is to neutralize pro-apoptotic BCL-2 family members. High MCL1 levels correlate with chemotherapy resistance and are a target for novel inhibitors.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8455/if_selected.jpg
Normal Tissue
Figure 2 Appendix
High expression in appendix
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8455/22739_A_1_2_val_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31125/65497_A_3_1_selected.jpg
Normal Tissue
Figure 4 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68195/ihc_selected.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31125/65497_A_7_3.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31125/65497_A_6_6.jpg
Normal Tissue
Figure 7 Thyroid gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31125/65497_B_3_5.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (U-698)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000143384-MCL1
❮
❯
Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Mouse MCL1
- Target
- MCL1
- Species Reactivity
- Mouse
- Isotype
- IgG
- Clone
- 01JF43
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-1025-YC75. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 01JF43"
- CAT
- Product Name
See other products for "MCL1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2309z | Mouse Anti-MCL1 Recombinant Antibody (clone 26F6) | WB, ELISA, IF, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1225CT | Recombinant Mouse anti-Human MCL1 Monoclonal antibody (49H4) | IHC-P |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2189 | Hi-Affi™ Recombinant Rabbit Anti-MCL1 Monoclonal Antibody (DS2189AB) | ICC, IF, WB, IHC-P, ICC, FC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0971-CN | Recombinant Rabbit Anti-MCL1 Monoclonal Antibody (CBACN-355) | WB, IF, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2028-CN | Rabbit Anti-MCL1 Polyclonal Antibody (MRO-2028-CN) | IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1025 | PE-A*03:01/Human MCL1 (RLLFFAPTR) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1026 | APC-A*03:01/Human MCL1 (RLLFFAPTR) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1027 | FITC-A*03:01/Human MCL1 (RLLFFAPTR) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1028 | A*03:01/Human MCL1 (RLLFFAPTR) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABS-0143 | Human Anti-MCL1 Recombinant Antibody (Clone 01JF43) | ELISA, FuncS | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBS-0143 | Human Anti-MCL1 Recombinant Antibody (Clone 01JF43); Fab Fragment | ELISA, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBS-0143 | Human Anti-MCL1 Recombinant Antibody (Clone 01JF43); scFv Fragment | ELISA, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1318 | Anti-MCL1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC710 | CytoStream™ Rabbit Anti-MCL1 Recombinant Antibody (VS13-YC710) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC402 | Recombinant Anti-MCL1 Vesicular Antibody, EV Displayed (VS-0425-YC402) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4301 | Anti-Mouse MCL1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4300 | Anti-Human MCL1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4302 | Anti-Rat MCL1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: Block, Cyt, FuncS, Inhib
Application: WB, ELISA, IF
Application: Block, IP, IF, FC
Application: ELISA, FC, IF, IHC, WB
-3.jpg)
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
