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    Mouse Anti-MECP2 Recombinant Antibody (clone 8H4A5B9) (CAT#: VS7-0425-WR376)

    This product is a mouse IgG1 antibody that binds to human MECP2. The antibody 8H4A5B9 recognizes specific amino acid domain of human MECP2 (AA: 7-148). It can be used for WB, IHC, ICC, and FC assays.

    Specific Inquiry
    • Size:

    • Conjugation:

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    • Purity:

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    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-251 MG
    Figure 1 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/593/if_selected.jpg
    Normal Tissue
    Figure 2 IHC staining of human cerebellum
    Figure 2 IHC staining of human cerebellum
    (Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells, cells in molecular layer and granular layer.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/37264/ihc_selected.jpg
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/593/1257_B_8_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Fibroblasts Staining: Medium Intensity: Moderate Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75% Peripheral nerve/ganglion Staining: High Intensity: Strong)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/593/1386_A_7_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/593/1386_A_8_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/593/1386_A_8_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/593/1386_A_6_6.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/593/1386_A_7_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Low cell line specificity
    Figure 9 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169057-MECP2

    Specifications

    • Immunogen
    • Recombinant human MECP2 (AA: 7-148) fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • Human MECP2
    • Species Reactivity
    • Human
    • Clone
    • 8H4A5B9
    • Molecule Class
    • Nuclear Protein
    • Applications
    • WB, IHC, ICC, FC
    • Conjugate
    • Unconjugated
    • Related Disease
    • Angelman syndrome (AS); Intellectual developmental disorder, X-linked, syndromic 13 (MRXS13); Rett syndrome (RTT); Autism, X-linked 3 (AUTSX3); Encephalopathy, neonatal severe, due to MECP2 mutations (ENS-MECP2); Intellectual developmental disorder, X-linked, syndromic, Lubs type (MRXSL)

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS
    • Preservative
    • 0.05% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:500 - 1:2000
      Immunohistochemistry-Paraffin: 1:200 - 1:1000
      Immunocytochemistry: 1:200 - 1:1000
      Flow Cytometry: 1:200 - 1:400
      Enzyme-linked Immunosorbent Assay: 1:10000

    Target

    • Alternative Names
    • MECP2; MRXS13; RS; AUTSX3; Methyl CpG Binding Protein 2 (Rett Syndrome); MRXSL; Mental Retardation, X-Linked 16; MeCp2; Methyl CpG Binding Protein 2; Mental Retardation, X-Linked 79; MeCp-2 Protein; Methyl-CpG-Binding Protein 2; Rett Syndrome; RTS; RTT; M
    • Long Name
    • Methyl Cpg Binding Protein 2 (Rett Syndrome)
    • Cellular Localization
    • Nucleus
    • Post Translation Modifications
    • Acetylation; Methylation; Phosphoprotein
    • Function
    • DNA-binding; Repressor

    Recommended Products

    Isotype Control

    Secondary Antibody

    Reconstitution Buffer

    Recommended Dilution Buffer

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "MECP2"

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    CAT Product Name Application Type
    MOB-4023z Mouse Anti-MECP2 Recombinant Antibody (clone 28F8) WB, ELISA, FC, IF, IHC Mouse IgG1
    MOB-1016CT Recombinant Mouse anti-Human MECP2 Monoclonal antibody (EML586) IHC-P, WB

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