Mouse Anti-MIB1 Recombinant Antibody (clone 6A9C9) (CAT#: VS7-0425-WR383)
This product is a mouse IgG1 antibody that binds to human MIB1. The antibody 6A9C9 recognizes specific amino acid domain of human MIB1 (AA: 6-221). It can be used for WB, ICC, and FC assays. It cross reacts with monkey.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19100/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19100/if_selected.jpg
Figure 2 IHC staining of human hippocampus
(Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19100/ihc_selected.jpg
(Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19100/ihc_selected.jpg
Figure 3 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_8_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_8_3.jpg
Figure 4 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_8_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_8_4.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_9_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_9_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_6_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_6_6.jpg
Figure 7 RNA cell line category: Cell line enhanced (REH)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101752-MIB1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101752-MIB1
Specifications
- Immunogen
- Recombinant human MIB1 (AA: 6-221) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MIB1
- Species Reactivity
- Human, Monkey
- Clone
- 6A9C9
- Molecule Class
- E3 Ubiquitin-Protein Ligase
- Applications
- WB, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Left ventricular non-compaction 7 (LVNC7)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MIB1; ZZZ6; Mindbomb E3 Ubiquitin Protein Ligase 1; LVNC7; ZZANK2; E3 Ubiquitin-Protein Ligase MIB1; MIB; DIP-1; DAPK-Interacting Protein 1; EC 6.3.2; EC 6.3.2.-; DIP1; Mindbomb Homolog 1 (Drosophila); Ubiquitin Ligase Mind Bomb; KIAA1323
- Gene ID
- 57534
- UniProt ID
- Q86YT6
- Long Name
- Mindbomb E3 Ubiquitin Protein Ligase 1
- Cellular Localization
- Cell membrane; Cytoplasm; Cytoskeleton; Membrane
- Post Translation Modifications
- Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_065825.1
- Function
- Transferase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "MIB1"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
Rabbit Monoclonal Antibody Products
Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2135z | Mouse Anti-MIB1 Recombinant Antibody (clone 32G7) | WB, ELISA, FC, ICC, IF | Mouse IgG1 |
| MRO-0998-CN | Recombinant Rabbit Anti-MIB1 Monoclonal Antibody (JG69-34) | WB, IF, IHC | Rabbit IgG |
| VS3-FY1770 | Rabbit Anti-MIB1 Recombinant Antibody (clone R06-3D9) | WB | Rabbit IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS7-0425-WR383. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: Block, IP, IF, FC
Application: ELISA, IHC, IF, IP, FC, FuncS
Application: Neut, IHC, Activ, FuncS, IF, ELISA
Application: ELISA, FC, Neut, Inhib
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
