Mouse Anti-MIB1 Recombinant Antibody (clone 6A9C9)
CAT#: VS7-0425-WR383
This product is a mouse IgG1 antibody that binds to human MIB1. The antibody 6A9C9 recognizes specific amino acid domain of human MIB1 (AA: 6-221). It can be used for WB, ICC, and FC assays. It cross reacts with monkey.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19100/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19100/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (REH)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101752-MIB1
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Specifications
- Immunogen
- Recombinant human MIB1 (AA: 6-221) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MIB1
- Species Reactivity
- Human, Monkey
- Clone
- 6A9C9
- Molecule Class
- E3 Ubiquitin-Protein Ligase
- Applications
- WB, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Left ventricular non-compaction 7 (LVNC7)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MIB1; ZZZ6; Mindbomb E3 Ubiquitin Protein Ligase 1; LVNC7; ZZANK2; E3 Ubiquitin-Protein Ligase MIB1; MIB; DIP-1; DAPK-Interacting Protein 1; EC 6.3.2; EC 6.3.2.-; DIP1; Mindbomb Homolog 1 (Drosophila); Ubiquitin Ligase Mind Bomb; KIAA1323
- Gene ID
- 57534
- UniProt ID
- Q86YT6
- Long Name
- Mindbomb E3 Ubiquitin Protein Ligase 1
- Cellular Localization
- Cell membrane; Cytoplasm; Cytoskeleton; Membrane
- Post Translation Modifications
- Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_065825.1
- Function
- Transferase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| MOB-2135z | Mouse Anti-MIB1 Recombinant Antibody (clone 32G7) | WB, ELISA, FC, ICC, IF | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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