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MIB1
Rabbit Anti-MIB1 Recombinant Antibody (clone R06-3D9)

Rabbit Anti-MIB1 Recombinant Antibody (clone R06-3D9) (CAT#: VS3-FY1770)

Name: Rabbit Anti-MIB1 Recombinant Antibody (clone R06-3D9)
SKU: VS3-FY1770
Rating: 5 (1 reviews)

This R06-3D9 recombinant monoclonal antibody was developed to identify human MIB1. It cross-reacts with mouse. The MIB1 antibody is recommended for detection of target protein by western blot analysis of Mib1/Mindbomb in K562, Hela lysates.

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Anti-MIB1 Antibody (VS3-FY1770) in WB.

Figure 1 Anti-MIB1 Antibody (VS3-FY1770) in WB.

The Mib1/Mindbomb expression in K562, Hela lysates were detected using the anti-MIB1 by western blot assay.

Subcellular Location

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19100/if_selected.jpg

Normal Tissue

Figure 2 IHC staining of human hippocampus
(Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19100/ihc_selected.jpg

Figure 3 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_8_3.jpg

Figure 4 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_8_4.jpg

Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_9_5.jpg

Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_6_6.jpg

RNA Expression

Figure 7 RNA cell line category: Cell line enhanced (REH)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101752-MIB1

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide of human Mib1

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human and Mouse MIB1

Species Reactivity
Human, Mouse

Clone
R06-3D9

Applications
Western Blot

MW
The calculated weight of the target protein is approximately 110 kDa, while the observed molecular weight stands at 110 kDa.

Related Disease
Left Ventricular Noncompaction 7 and Left Ventricular Noncompaction

Product Property

Purification
Affinity Purified

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000

Target

Alternative Names
Mindbomb E3 Ubiquitin Protein Ligase 1; Zinc Finger ZZ Type With Ankyrin Repeat Domain Protein 2; RING-Type E3 Ubiquitin Transferase MIB1; DAPK-Interacting Protein 1; ZZANK2; DIP-1; DIP1; E3 Ubiquitin-Protein Ligase MIB1; Mindbomb Homolog 1 (Drosophila)

Gene ID
57534

UniProt ID
Q86YT6

Cellular Localization
Cell membrane, Cytoplasm, Cytoskeleton, Membrane

Post Translation Modifications
Ubiquitinated; possibly via autoubiquitination (By similarity).
Ubiquitinated; this modification is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock.

Protein Refseq
XP_011524400.1; XP_016881363.1; XP_016881364.1; XP_047293632.1; XP_054174842.1

Function
E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis (By similarity).

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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