Rabbit Anti-MIB1 Recombinant Antibody (clone R06-3D9)
CAT#: VS3-FY1770
This R06-3D9 recombinant monoclonal antibody was developed to identify human MIB1. It cross-reacts with mouse. The MIB1 antibody is recommended for detection of target protein by western blot analysis of Mib1/Mindbomb in K562, Hela lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19100/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19100/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37044/78468_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (REH)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101752-MIB1
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WB
Figure 1 Anti-MIB1 Antibody (VS3-FY1770) in WB.
The Mib1/Mindbomb expression in K562, Hela lysates were detected using the anti-MIB1 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human Mib1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse MIB1
- Species Reactivity
- Human, Mouse
- Clone
- R06-3D9
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 110 kDa, while the observed molecular weight stands at 110 kDa.
- Related Disease
- Left Ventricular Noncompaction 7 and Left Ventricular Noncompaction
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Mindbomb E3 Ubiquitin Protein Ligase 1; Zinc Finger ZZ Type With Ankyrin Repeat Domain Protein 2; RING-Type E3 Ubiquitin Transferase MIB1; DAPK-Interacting Protein 1; ZZANK2; DIP-1; DIP1; E3 Ubiquitin-Protein Ligase MIB1; Mindbomb Homolog 1 (Drosophila)
- Gene ID
- 57534
- UniProt ID
- Q86YT6
- Cellular Localization
- Cell membrane, Cytoplasm, Cytoskeleton, Membrane
- Post Translation Modifications
- Ubiquitinated; possibly via autoubiquitination (By similarity).
Ubiquitinated; this modification is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock.
- Protein Refseq
- XP_011524400.1; XP_016881363.1; XP_016881364.1; XP_047293632.1; XP_054174842.1
- Function
- E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| MOB-2135z | Mouse Anti-MIB1 Recombinant Antibody (clone 32G7) | WB, ELISA, FC, ICC, IF | Mouse IgG1 |
| MRO-0998-CN | Recombinant Rabbit Anti-MIB1 Monoclonal Antibody (JG69-34) | WB, IF, IHC | Rabbit IgG |
| VS7-0425-WR383 | Mouse Anti-MIB1 Recombinant Antibody (clone 6A9C9) | WB, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2230 | Hi-Affi™ Recombinant Rabbit Anti-MIB1 Monoclonal Antibody (DS2230AB) | WB, IHC, ICC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY323 | Mouse Anti-NHP MIB1 Recombinant Antibody (clone 2A7B1) | WB, FC, ELISA | Mouse IgG1 |
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