Rabbit Anti-MIB1 Recombinant Antibody (clone R06-3D9) (CAT#: VS3-FY1770)
This R06-3D9 recombinant monoclonal antibody was developed to identify human MIB1. It cross-reacts with mouse. The MIB1 antibody is recommended for detection of target protein by western blot analysis of Mib1/Mindbomb in K562, Hela lysates.
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WB
Figure 1 Anti-MIB1 Antibody (VS3-FY1770) in WB.
The Mib1/Mindbomb expression in K562, Hela lysates were detected using the anti-MIB1 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human Mib1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse MIB1
- Species Reactivity
- Human, Mouse
- Clone
- R06-3D9
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 110 kDa, while the observed molecular weight stands at 110 kDa.
- Related Disease
- Left Ventricular Noncompaction 7 and Left Ventricular Noncompaction
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Mindbomb E3 Ubiquitin Protein Ligase 1; Zinc Finger ZZ Type With Ankyrin Repeat Domain Protein 2; RING-Type E3 Ubiquitin Transferase MIB1; DAPK-Interacting Protein 1; ZZANK2; DIP-1; DIP1; E3 Ubiquitin-Protein Ligase MIB1; Mindbomb Homolog 1 (Drosophila)
- Gene ID
- 57534
- UniProt ID
- Q86YT6
- Cellular Localization
- Cell membrane, Cytoplasm, Cytoskeleton, Membrane
- Post Translation Modifications
- Ubiquitinated; possibly via autoubiquitination (By similarity).
Ubiquitinated; this modification is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock.
- Protein Refseq
- XP_011524400.1; XP_016881363.1; XP_016881364.1; XP_047293632.1; XP_054174842.1
- Function
- E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "MIB1"
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2135z | Mouse Anti-MIB1 Recombinant Antibody (clone 32G7) | WB, ELISA, FC, ICC, IF | Mouse IgG1 |
| MRO-0998-CN | Recombinant Rabbit Anti-MIB1 Monoclonal Antibody (JG69-34) | WB, IF, IHC | Rabbit IgG |
| VS7-0425-WR383 | Mouse Anti-MIB1 Recombinant Antibody (clone 6A9C9) | WB, ICC, FC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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