Mouse Anti-MKRN2 Recombinant Antibody (VS3-FY2803)
CAT#: VS3-FY2803
This anti-MKRN2 antibody is a mouse recombinant monoclonal antibody that is targeting for human MKRN2 protein. It exhibits cross-reactivity with mouse, rat species. The calculated molecular weight of the protein is about 46.9 kDa. This monoclonal antibody proves valuable in disease research related to Oligoasthenoteratozoospermia. The antibody can be utilized for detection of MKRN2 by Western Blot (1/500-1/2000), Immunohistochemistry (1/50-1/200), Immunocytochemistry (1/10-1/25) and Flow cytometry (1/10-1/25).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37559/430_G4_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/430_H1_2_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/76294_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/76294_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/76294_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/76294_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/76294_A_6_6.jpg
Normal Tissue
Figure 8 Heart muscle
Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/76294_B_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000075975-MKRN2
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse and Rat MKRN2
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Western Blot, Immunohistochemistry, Immunocytochemistry, Flow cytometry
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 46.9 kDa.
- Related Disease
- Oligoasthenoteratozoospermia
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Immunocytochemistry: 1/10-1/25
Flow cytometry: 1/10-1/25
Target
- Alternative Names
- Makorin Ring Finger Protein 2; RING-Type E3 Ubiquitin Transferase Makorin-2; RING Finger Protein 62; RNF62; Probable E3 Ubiquitin-Protein Ligase Makorin-2; Makorin RING Zinc-Finger Protein 2; EC 2.3.2.27; HSPC070
- Gene ID
- 23609
- UniProt ID
- Q9H000
- Cellular Localization
- Cytoplasm
Nucleus
- Post Translation Modifications
- Ubiquitination at Lys42 and Lys334 (NX_Q9H000 [NX_Q9H000-1])
Modification sites at PhosphoSitePlus (Q9H000)
Glycosylation from GlyGen (Q9H000) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_054879.3
- Function
- E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (By similarity).
Promotes the polyubiquitination and proteasome-dependent degradation of RELA/p65, thereby suppressing RELA-mediated NF-kappaB transactivation and negatively regulating inflammatory responses (By similarity).
Plays a role in the regulation of spermiation and in male fertility (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MKRN2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2042-CN | Rabbit Anti-MKRN2 Polyclonal Antibody (MRO-2042-CN) | WB, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK1143 | Mouse Anti-MKRN2 Recombinant Antibody (VS3-WK1143) | WB, IHC, FC | Mouse IgG |
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