Mouse Anti-MKRN2 Recombinant Antibody (VS3-FY2803) (CAT#: VS3-FY2803)
This anti-MKRN2 antibody is a mouse recombinant monoclonal antibody that is targeting for human MKRN2 protein. It exhibits cross-reactivity with mouse, rat species. The calculated molecular weight of the protein is about 46.9 kDa. This monoclonal antibody proves valuable in disease research related to Oligoasthenoteratozoospermia. The antibody can be utilized for detection of MKRN2 by Western Blot (1/500-1/2000), Immunohistochemistry (1/50-1/200), Immunocytochemistry (1/10-1/25) and Flow cytometry (1/10-1/25).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37559/430_G4_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37559/430_G4_2_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/430_H1_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/430_H1_2_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/76294_B_9_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/76294_B_9_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/76294_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/76294_A_7_3.jpg
Figure 5 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/76294_A_8_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/76294_A_8_4.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/76294_A_8_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/76294_A_8_5.jpg
Figure 7 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/76294_A_6_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/76294_A_6_6.jpg
Figure 8 Heart muscle
(Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/76294_B_4_6.jpg
(Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37560/76294_B_4_6.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000075975-MKRN2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000075975-MKRN2
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse and Rat MKRN2
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Western Blot, Immunohistochemistry, Immunocytochemistry, Flow cytometry
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 46.9 kDa.
- Related Disease
- Oligoasthenoteratozoospermia
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Immunocytochemistry: 1/10-1/25
Flow cytometry: 1/10-1/25
Target
- Alternative Names
- Makorin Ring Finger Protein 2; RING-Type E3 Ubiquitin Transferase Makorin-2; RING Finger Protein 62; RNF62; Probable E3 Ubiquitin-Protein Ligase Makorin-2; Makorin RING Zinc-Finger Protein 2; EC 2.3.2.27; HSPC070
- Gene ID
- 23609
- UniProt ID
- Q9H000
- Cellular Localization
- Cytoplasm
Nucleus
- Post Translation Modifications
- Ubiquitination at Lys42 and Lys334 (NX_Q9H000 [NX_Q9H000-1])
Modification sites at PhosphoSitePlus (Q9H000)
Glycosylation from GlyGen (Q9H000) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_054879.3
- Function
- E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (By similarity).
Promotes the polyubiquitination and proteasome-dependent degradation of RELA/p65, thereby suppressing RELA-mediated NF-kappaB transactivation and negatively regulating inflammatory responses (By similarity).
Plays a role in the regulation of spermiation and in male fertility (By similarity).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2042-CN | Rabbit Anti-MKRN2 Polyclonal Antibody (MRO-2042-CN) | WB, IF, IHC | Rabbit IgG |
| VS3-WK1143 | Mouse Anti-MKRN2 Recombinant Antibody (VS3-WK1143) | WB, IHC, FC | Mouse IgG |
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