Anti-Mouse EPHB2 Immunohistochemistry Kit
CAT#: VS-0525-XY2315
This EPHB2 IHC kit offers a complete solution for detecting Eph receptor B2 in tissue sections. The kit works efficiently with both paraffin and frozen tissue sections, delivering high sensitivity, clear results, and reproducibility with minimal background interference.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71200/1400_A5_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13647/31121_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13647/31121_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13647/31121_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13647/31121_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13647/31121_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13647/31121_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (GAMG, HUVEC TERT2, TIME, U-251 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000133216-EPHB2
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Mouse
- Target
- EPHB2
- Primary Antibody
- Rat Anti-EPHB2 Antibody
- Secondary Antibody
- Goat anti-Rat Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| MOB-0657CT | Recombinant Mouse anti-Human EPHB2 Monoclonal antibody (EML072) | WB | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0292-CN-S(P) | Mouse Anti-EPHB2 Recombinant Antibody; scFv Fragment (HPAB-0292-CN-S(P)) | ELISA, WB, FC, IHC, IF, IP | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0292-CN-F(E) | Mouse Anti-EPHB2 Recombinant Antibody; Fab Fragment (HPAB-0292-CN-F(E)) | ELISA, WB, FC, IHC, IF, IP | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY36 | Mouse Anti-EPHB2 (clone 2H9) scFv-Fc Chimera | ELISA, IHC, FC, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC585 | Recombinant Anti-EPHB2 Vesicular Antibody, EV Displayed (VS-0425-YC585) | ELISA, FC, Cell-uptake |
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