Mouse Anti-EPHB2 (clone 2H9) scFv-Fc Chimera
CAT#: VS-0425-FY36
Anti-EPHB2 scFv-Fc is a recombinant antibody designed to target the EPHB2 receptor tyrosine kinase. 1 This format combines the antigen-binding specificity of a single-chain variable fragment (scFv) with the stability, potential for dimerization, and effector functions of an immunoglobulin Fc region. It is being developed as a potential tool for research and therapeutic applications, aiming to modulate EPHB2 signaling involved in processes like cell motility, angiogenesis, and tumor progression. The Fc region may also contribute to extended half-life and potential engagement of immune effector mechanisms.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71200/1400_A5_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13647/31121_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13647/31121_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13647/31121_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13647/31121_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13647/31121_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13647/31121_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (GAMG, HUVEC TERT2, TIME, U-251 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000133216-EPHB2
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Specifications
- Immunogen
- Baculovirus-derived His8-tagged EphB2 receptor.
- Host Species
- Mouse
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human EPHB2
- Species Reactivity
- Human, Mouse
- Clone
- 2H9
- Applications
- ELISA, Immunohistochemistry, Flow Cytometry, Inhibition
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Immunohistochemistry: 5 μg/mL
Flow Cytometry: 1 μg/mL
The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- EPH Receptor B2; Developmentally-Regulated Eph-Related Tyrosine Kinase; Tyrosine-Protein Kinase Receptor EPH-3; Renal Carcinoma Antigen NY-REN-47; Tyrosine-Protein Kinase TYRO5; EPH-Like Kinase 5; EC 2.7.10.1; Tyro5; EPHT3; Hek5; DRT; ERK; EK5
- Gene ID
- 2048
- UniProt ID
- P29323
- Long Name
- EPH Receptor B2
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Autophosphorylated; ligand binding stimulates autophosphorylation on tyrosine residues.
Polyubiquitinated; ligand binding stimulates ubiquitination.
Ubiquitinated by RNF186 at Lys-891, mainly through 'Lys-27'-linked polyubiquitin chains.
Ubiquitinated by CRL2 E3 ligase complex.
- Protein Refseq
- XP_006710504.1; XP_006710505.1; XP_047305060.1; XP_054191013.1; XP_054191014.1
- Function
- Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells.
The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.
Functions in axon guidance during development.
Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex.
Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk.
In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses.
Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation.
Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0657CT | Recombinant Mouse anti-Human EPHB2 Monoclonal antibody (EML072) | WB | |
| HPAB-0292-CN | Mouse Anti-EPHB2 Recombinant Antibody (HPAB-0292-CN) | ELISA, WB, FC, IHC, IF, IP | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0292-CN-S(P) | Mouse Anti-EPHB2 Recombinant Antibody; scFv Fragment (HPAB-0292-CN-S(P)) | ELISA, WB, FC, IHC, IF, IP | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0292-CN-F(E) | Mouse Anti-EPHB2 Recombinant Antibody; Fab Fragment (HPAB-0292-CN-F(E)) | ELISA, WB, FC, IHC, IF, IP | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC585 | Recombinant Anti-EPHB2 Vesicular Antibody, EV Displayed (VS-0425-YC585) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
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| VS-0525-XY2315 | Anti-Mouse EPHB2 Immunohistochemistry Kit | IHC |
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