Anti-Mouse GLI1 Immunohistochemistry Kit
CAT#: VS-0525-XY2823
The GLI1 IHC kit provides complete reagents for detecting this transcription factor involved in the Hedgehog signaling pathway. Suitable for paraffin and frozen tissue sections across species, it supports research on developmental biology, cancer progression, and stem cell regulation with reliable and consistent staining.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65172/1154_H2_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68903/1609_F8_2_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9460/991_G2_2_selected.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9460/27244_A_6_6.jpg
Normal Tissue
Figure 5 Cerebellum
Purkinje cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9460/27244_B_7_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (AN3-CA, BJ hTERT+, NTERA-2, RH-30, U-266/70)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111087-GLI1
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse, Rat
- Target
- GLI1
- Primary Antibody
- Mouse Anti-GLI1 Antibody
- Secondary Antibody
- Goat anti-Mouse Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Related Signaling Pathways
Hedgehog Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "GLI1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4082z | Mouse Anti-GLI1 Recombinant Antibody (clone 16A6) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
| MRO-0658-CN | Rabbit Anti-GLI1 Recombinant Antibody (clone JF09-08) | WB, IF, IHC | Rabbit IgG |
| VS3-WK93 | Mouse Anti-GLI1 Recombinant Antibody (clone F8-B9) | WB, ELISA | Mouse IgG1 |
| VS7-HM1006 | Mouse Anti-GLI1 Recombinant Antibody (clone 1B9F8) | IHC, ICC, FCM | Mouse IgG1 |
| VS13-YC451 | Rabbit Anti-GLI1 Recombinant Antibody (VS13-YC451) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1451 | Hi-Affi™ Rabbit Anti-GLI1 Recombinant Antibody (clone DS1451AB) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2822 | Anti-GLI1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2824 | Anti-Rat GLI1 Immunohistochemistry Kit | IHC |
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