Mouse Anti-MPO Recombinant Antibody (clone 6D6) (CAT#: FAMAB-0622WJ)
This product is a mouse anti-myeloperoxidase (MPO) monoclonal antibody. The 6D6 bounds to the native form of MPO and had a high constant affinity for MPO as demonstrated by real-time specific interaction.
Specific Inquiry
ELISA
Figure 1 Binding activity of monoclonal antibody 6D6.
Binding activity of monoclonal antibody 9B5 (a) and 6D6 (b) detected by solid-phase enzyme-linked immunosorbent assay using myeloperoxidase (○), myosin (•), rabbit IgG (▿), proteinase 3 (▾), ssDNA and histones (□) as the antigen.
Vittecoq, O., Brard, F., Jovelin, F., Le Loet, X., Tron, F., & Gilbert, D. (1999). IgM anti-myeloperoxidase antibody-secreting lymphocytes are present in the peripheral repertoire of lupus mice but rarely differentiate into IgG-producing cells. Clinical & Experimental Immunology, 118(1), 122-130.
Inhib
Figure 2 Inhibition of anti-myeloperoxidase activity of monoclonal antibody 6D6 (•) and 9B5 (○) by various concentrations of myeloperoxidase.
Vittecoq, O., Brard, F., Jovelin, F., Le Loet, X., Tron, F., & Gilbert, D. (1999). IgM anti-myeloperoxidase antibody-secreting lymphocytes are present in the peripheral repertoire of lupus mice but rarely differentiate into IgG-producing cells. Clinical & Experimental Immunology, 118(1), 122-130.
IF
Figure 3 Immunofluorescence staining patterns given by monoclonal antibody 6D6 (a,c) and 9B5 (b,d).
Immunofluorescence staining patterns given by monoclonal antibody 6D6 (a,c) and 9B5 (b,d) tested on ethanol-fixed human neutrophils (a,b) using an indirect immunofluorescence assay (magnification ×400), or mouse white blood cells (c,d).
Vittecoq, O., Brard, F., Jovelin, F., Le Loet, X., Tron, F., & Gilbert, D. (1999). IgM anti-myeloperoxidase antibody-secreting lymphocytes are present in the peripheral repertoire of lupus mice but rarely differentiate into IgG-producing cells. Clinical & Experimental Immunology, 118(1), 122-130.
SPR
Figure 4 Overlay plots illustrating the binding of monoclonal antibody 6D6 and 9B5 at the concentration of 25 nm to immobilized myeloperoxidase.
A flow-rate of 5 μl/min was used and for each curve, 15 μl of antibody were injected.
Vittecoq, O., Brard, F., Jovelin, F., Le Loet, X., Tron, F., & Gilbert, D. (1999). IgM anti-myeloperoxidase antibody-secreting lymphocytes are present in the peripheral repertoire of lupus mice but rarely differentiate into IgG-producing cells. Clinical & Experimental Immunology, 118(1), 122-130.
WB
Figure 5 Western blot analysis of monoclonal antibody (mAb) 6D6 and 9B5.
Western blot analysis of monoclonal antibody (mAb) 6D6 and 9B5 using nonreduced (lanes 1 and 2) and reduced myeloperoxidase (lanes 3 and 4). Molecular weight markers are indicated on the left hand part of the figure. Lanes 1 and 3: mAb 6D6; lanes 2 and 4: mAb 9B5.
Vittecoq, O., Brard, F., Jovelin, F., Le Loet, X., Tron, F., & Gilbert, D. (1999). IgM anti-myeloperoxidase antibody-secreting lymphocytes are present in the peripheral repertoire of lupus mice but rarely differentiate into IgG-producing cells. Clinical & Experimental Immunology, 118(1), 122-130.
Specifications
- Host Species
- Mouse
- Derivation
- Hybridoma
- Type
- Mouse IgM, κ
- Specificity
- Human MPO
- Species Reactivity
- Human
- Clone
- 6D6
- Applications
- ELISA, WB, IF
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Myeloperoxidase; MPO
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Antibody Products
IgG Antibody Products
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ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1015 | Recombinant Mouse Anti-Human MPO Antibody Fab Fragment | LMNX, ELISA | Fab |
| FAMAB-0256WJ-F(E) | Human Anti-MPO Recombinant Antibody (clone E3-MPO); Fab Fragment | ELISA, WB | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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