CytoStream™ Mouse Anti-MPO Recombinant Antibody (VS13-YC738) (CAT#: VS13-YC738)
The CytoStream™ Mouse Anti-MPO Recombinant Antibody is specific for human, mouse and rat MPO. MPO encodes a heme protein central to neutrophil's microbicidal activity, synthesizing hypohalous acids, with a mature tetrameric structure. The anti-MPO antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1487_B11_3_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1487_B11_3_selected.jpg
Figure 2 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1403_F7_1_red_green.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1403_F7_1_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1398_F7_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1398_F7_1_red_green.jpg
Figure 4 Bone marrow
(Hematopoietic cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/145726_B_6_4.jpg
(Hematopoietic cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/145726_B_6_4.jpg
Figure 5 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21147/146573_A_9_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21147/146573_A_9_8.jpg
Figure 6 RNA cell line category: Group enriched (HL-60, NB-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000005381-MPO
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000005381-MPO
Figure 6 RNA cell line category: Group enriched (HL-60, NB-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000005381-MPO
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000005381-MPO
Specifications
- Immunogen
- Recombinant human Myeloperoxidase, aa 550-745.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human, Mouse and Rat MPO
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 50% Glycerol and 0.2% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/2000-1/5000
Immunohistochemistry-paraffin (IHC-P): 1/5000-1/10000
Flow cytometry (FC): 1/50-1/100
Immunofluorescence (IF-cell): 1/100
Immunofluorescence (IF-tissue): 1/1000-1/2000
Target
- Alternative Names
- Myeloperoxidase; EC 1.11.1.7; EC 1.11.2.2; EC 1.11.1;
- Gene ID
- 4353
- UniProt ID
- P05164
- Long Name
- Myeloperoxidase
- Cellular Localization
- Lysosome
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1015 | Recombinant Mouse Anti-Human MPO Antibody Fab Fragment | LMNX, ELISA | Fab |
| FAMAB-0256WJ-F(E) | Human Anti-MPO Recombinant Antibody (clone E3-MPO); Fab Fragment | ELISA, WB | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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