CytoStream™ Mouse Anti-MPO Recombinant Antibody (VS13-YC738)
CAT#: VS13-YC738
The CytoStream™ Mouse Anti-MPO Recombinant Antibody is specific for human, mouse and rat MPO. MPO encodes a heme protein central to neutrophil's microbicidal activity, synthesizing hypohalous acids, with a mature tetrameric structure. The anti-MPO antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1487_B11_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1403_F7_1_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1398_F7_1_red_green.jpg
Normal Tissue
Figure 4 Bone marrow
Hematopoietic cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/145726_B_6_4.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21147/146573_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (HL-60, NB-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000005381-MPO
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Specifications
- Immunogen
- Recombinant human Myeloperoxidase, aa 550-745.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human, Mouse and Rat MPO
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 50% Glycerol and 0.2% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/2000-1/5000
Immunohistochemistry-paraffin (IHC-P): 1/5000-1/10000
Flow cytometry (FC): 1/50-1/100
Immunofluorescence (IF-cell): 1/100
Immunofluorescence (IF-tissue): 1/1000-1/2000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MPO"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1015 | Recombinant Mouse Anti-Human MPO Antibody Fab Fragment | LMNX, ELISA | Fab |
| FAMAB-0256WJ-F(E) | Human Anti-MPO Recombinant Antibody (clone E3-MPO); Fab Fragment | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2273 | Hi-Affi™ Recombinant Rabbit Anti-MPO Monoclonal Antibody (DS2273AB) | ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0256WJ-S(P) | Human Anti-MPO Recombinant Antibody (clone E3-MPO); scFv Fragment | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0622WJ | Mouse Anti-MPO Recombinant Antibody (clone 6D6) | ELISA, WB, IF | Mouse IgM, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0525F | Mouse Anti-MPO Recombinant Antibody (clone 8E6) | FC | Mouse IgG1 |
| ZG-0529F | Mouse Anti-MPO Antibody (clone 8E6 and RFB4) | FC | Mouse IgG1 |
| VS3-XY1095 | Mouse Anti-MPO Recombinant Antibody, FITC (clone 4AMPO455-8E6) | FC, IF | Mouse IgG1 |
| VS3-XY1096 | Mouse Anti-MPO Recombinant Antibody (clone 4AMPO455-8E6) | FC, IF | Mouse IgG1 |
| VS3-XY1097 | Mouse Anti-MPO Recombinant Antibody (clone 2G3) | WB, IP, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY164 | CytoStream™ Mouse Anti-MPO Recombinant Antibody (clone 8F4/MPO) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1365 | Anti-MPO Immunohistochemistry Kit | IHC | |
| VS-0525-XY4482 | Anti-Mouse MPO Immunohistochemistry Kit | IHC | |
| VS-0525-XY4481 | Anti-Human MPO Immunohistochemistry Kit | IHC | |
| VS-0525-XY4483 | Anti-Rat MPO Immunohistochemistry Kit | IHC |
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