Human Anti-MPO Recombinant Antibody (clone E3-MPO) (CAT#: FAMAB-0256WJ)
This product is a human monoclonal anti-MPO antibody (E3-MPO) generated using peripheral blood lymphocytes from a patient with microscopic polyarteritis. This antibody can be used in applications, such as ELISA, WB.
Specific Inquiry
ELISA
Figure 1 A syk family kinase and a pertussis sensitive G-protein mediate the effect of E3MPO on neutrophil adhesion.
Preloading of neutrophils with the syk-family kinase inhibitor piceatannol (Pic) (100 nM for 1 h) increased neutrophil binding to plastic in the presence of E3-MPO by over five-fold, neutralizing the inhibitory effect of the antibody.
Hayes, M. J., & Cambridge, G. (2004). An IgM class anti-neutrophil cytoplasm antibody inhibits neutrophil adhesion and apoptosis via a Syk dependent signaling cascade. Molecular immunology, 41(4), 457–468.
IF
Figure 1 Indirect Immunofluorescence of MPO
Indirect Immunofluorescence of MPO A and D (stained with E3-MPO) and CD18 B and E reveals co-localization on the surface of freshlypurified neutrophils C and F.
Hayes, M. J., & Cambridge, G. (2004). An IgM class anti-neutrophil cytoplasm antibody inhibits neutrophil adhesion and apoptosis via a Syk dependent signaling cascade. Molecular immunology, 41(4), 457–468.
Specifications
- Host Species
- Human
- Derivation
- Hybridoma
- Type
- Human IgG
- Specificity
- Myeloperoxidase
- Species Reactivity
- Human
- Clone
- E3-MPO
- Applications
- ELISA, WB
- Related Disease
- vasculitis
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone E3-MPO"
See other products for "MPO"
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1015 | Recombinant Mouse Anti-Human MPO Antibody Fab Fragment | LMNX, ELISA | Fab |
| FAMAB-0256WJ-F(E) | Human Anti-MPO Recombinant Antibody (clone E3-MPO); Fab Fragment | ELISA, WB | Human Fab |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2273 | Hi-Affi™ Recombinant Rabbit Anti-MPO Monoclonal Antibody (DS2273AB) | ICC, IHC-P, WB | IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0256WJ-S(P) | Human Anti-MPO Recombinant Antibody (clone E3-MPO); scFv Fragment | ELISA, WB | Human scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0129F | Mouse Anti-MPO Recombinant Antibody (MOB-0129F) | IHC-P, IF | Mouse IgG |
| MOB-0130F | Mouse Anti-MPO Recombinant Antibody (MOB-0130F) | IHC-P, IF | Mouse IgG |
| ZG-0288C | Mouse Anti-MPO Recombinant Antibody (ZG-0288C) | WB, ELISA | Mouse IgG |
| VS3-FY942 | Recombinant Mouse Anti-MPO Antibody (clone 9D10-2F3-3E7) | IHC-P | Mouse IgG1 |
| VS3-FY943 | Recombinant Rabbit Anti-MPO Antibody (clone R03-6F2) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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