Human Anti-MPO Recombinant Antibody (clone E3-MPO)
CAT#: FAMAB-0256WJ Autoantibody
This product is a human monoclonal anti-MPO antibody (E3-MPO) generated using peripheral blood lymphocytes from a patient with microscopic polyarteritis. This antibody can be used in applications, such as ELISA, WB.
Gene Expression
Published Data
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1487_B11_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1403_F7_1_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1398_F7_1_red_green.jpg
Normal Tissue
Figure 4 Bone marrow
Hematopoietic cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/145726_B_6_4.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21147/146573_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (HL-60, NB-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000005381-MPO
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ELISA
Figure 1 A syk family kinase and a pertussis sensitive G-protein mediate the effect of E3MPO on neutrophil adhesion.
Preloading of neutrophils with the syk-family kinase inhibitor piceatannol (Pic) (100 nM for 1 h) increased neutrophil binding to plastic in the presence of E3-MPO by over five-fold, neutralizing the inhibitory effect of the antibody.
Hayes, M. J., & Cambridge, G. (2004). An IgM class anti-neutrophil cytoplasm antibody inhibits neutrophil adhesion and apoptosis via a Syk dependent signaling cascade. Molecular immunology, 41(4), 457–468.
IF
Figure 1 Indirect Immunofluorescence of MPO
Indirect Immunofluorescence of MPO A and D (stained with E3-MPO) and CD18 B and E reveals co-localization on the surface of freshlypurified neutrophils C and F.
Hayes, M. J., & Cambridge, G. (2004). An IgM class anti-neutrophil cytoplasm antibody inhibits neutrophil adhesion and apoptosis via a Syk dependent signaling cascade. Molecular immunology, 41(4), 457–468.
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Specifications
- Host Species
- Human
- Derivation
- Hybridoma
- Type
- Human IgG
- Specificity
- Myeloperoxidase
- Species Reactivity
- Human
- Clone
- E3-MPO
- Applications
- ELISA, WB
- Related Disease
- vasculitis
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone E3-MPO"
- CAT
- Product Name
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Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1015 | Recombinant Mouse Anti-Human MPO Antibody Fab Fragment | LMNX, ELISA | Fab |
| FAMAB-0256WJ-F(E) | Human Anti-MPO Recombinant Antibody (clone E3-MPO); Fab Fragment | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0004MZ | Recombinant Mouse Anti-Human Myeloperoxidase Antibody (clone NPO-8) | ELISA | Mouse antibody |
| MRO-1053-CN | Recombinant Rabbit Anti-MPO Monoclonal Antibody (JM10-58) | WB, IF, IHC | Rabbit IgG |
| ZG-0525F | Mouse Anti-MPO Recombinant Antibody (clone 8E6) | FC | Mouse IgG1 |
| ZG-0529F | Mouse Anti-MPO Antibody (clone 8E6 and RFB4) | FC | Mouse IgG1 |
| ZG-0530F | Mouse Anti-MPO Antibody (clone 8E6 and UCHT1) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2273 | Hi-Affi™ Recombinant Rabbit Anti-MPO Monoclonal Antibody (DS2273AB) | ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0256WJ-S(P) | Human Anti-MPO Recombinant Antibody (clone E3-MPO); scFv Fragment | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0622WJ | Mouse Anti-MPO Recombinant Antibody (clone 6D6) | ELISA, WB, IF | Mouse IgM, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY164 | CytoStream™ Mouse Anti-MPO Recombinant Antibody (clone 8F4/MPO) | FC | Mouse IgG1, kappa |
| VS13-YC738 | CytoStream™ Mouse Anti-MPO Recombinant Antibody (VS13-YC738) | WB, IHC-P, ICC, IF, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1365 | Anti-MPO Immunohistochemistry Kit | IHC | |
| VS-0525-XY4482 | Anti-Mouse MPO Immunohistochemistry Kit | IHC | |
| VS-0525-XY4481 | Anti-Human MPO Immunohistochemistry Kit | IHC | |
| VS-0525-XY4483 | Anti-Rat MPO Immunohistochemistry Kit | IHC |
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