Mouse Anti-MSH2 Recombinant Antibody (VS3-CJ961)
CAT#: VS3-CJ961
This product is a mouse antibody that recognizes human MSH2.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66845/1768_C3_11_cr594bb51d24b1e_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9572/if_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9572/26473_A_9_8.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9572/26473_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9572/26473_A_7_5.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000095002-MSH2
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Specifications
- Immunogen
- KLH-conjugated synthetic peptide encompassing a sequence within human MSH2
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MSH2
- Species Reactivity
- Human
- Applications
- WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.2% BSA, PBS.
- Preservative
- 0.01% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:1000), Immunohistochemistry (1:100-1:300).
Target
- Alternative Names
- FCC1; COCA1; HNPCC; LCFS2; MSH-2; hMSH2; HNPCC1; MMRCS2
- Gene ID
- 4436
- UniProt ID
- P43246
- Sequence Similarities
- Belongs to the DNA mismatch repair MutS family.
- Cellular Localization
- Chromosome, Nucleus
- Post Translation Modifications
- Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.
- Protein Refseq
- NP_000242.1; NP_001245210.1
- Function
- Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand (PubMed:26300262).
ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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|---|---|---|---|
| MOB-0922MZ | Recombinant Mouse Anti-Human MSH2 Antibody (clone 36E23) | IHC-Fr, WB | Mouse antibody |
| MRO-1026-CN | Recombinant Mouse Anti-MSH2 Monoclonal Antibody (10G1) | WB, IHC | Mouse IgG2b |
| MRO-1027-CN | Recombinant Mouse Anti-MSH2 Monoclonal Antibody (10G2) | WB, IHC | Mouse IgG2b |
| MRO-1028-CN | Recombinant Mouse Anti-MSH2 Monoclonal Antibody (10G3) | WB, IHC | Mouse IgG2b |
| ZG-0289C | Mouse Anti-MSH2 Recombinant Antibody (ZG-0289C) | WB, IHC, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2297 | Hi-Affi™ Recombinant Rabbit Anti-MSH2 Monoclonal Antibody (DS2297AB) | FC, ICC, IF, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC845 | AbPlus™ Anti-MSH2 Magnetic Beads (VS-0724-YC845) | IP, Protein Purification |
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