Rabbit Anti-MTAP Recombinant Antibody (clone R07-5A0) (CAT#: VS3-FY2040)
The R07-5A0 recombinant monoclonal antibody is capable of recognizing human MTAP. It cross-reacts with mouse. The antibody has the positive detection of MTAP protein by western blot analysis of MTAP in 3T3 lysates and western blot analysis of MTAP in A549, MCF-7, U251, HL-60 lysates.
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WB
Figure 1 Anti-MTAP Antibody (VS3-FY2040) in WB.
The MTAP expression in 3T3 lysates were detected using the anti-MTAP by western blot assay.
WB
Figure 2 Anti-MTAP Antibody (VS3-FY2040) in WB.
The MTAP expression in A549, MCF-7, U251, HL-60 lysates were detected using the anti-MTAP by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human MTAP
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse MTAP
- Species Reactivity
- Human, Mouse
- Clone
- R07-5A0
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 31 kDa, while the observed molecular weight stands at 31 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Methylthioadenosine Phosphorylase; S-Methyl-5-Thioadenosine Phosphorylase; 5-Methylthioadenosine Phosphorylase; MTA Phosphorylase; MTAPase; MSAP; Epididymis Luminal Protein 249; MeSAdo Phosphorylase
- Gene ID
- 4507
- UniProt ID
- Q13126
- Sequence Similarities
- Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys40 and Lys71 (NX_Q13126 [NX_Q13126-1])
Modification sites at PhosphoSitePlus (Q13126)
Glycosylation from GlyGen (Q13126) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001382969.1; NP_001382970.1; NP_001382971.1; NP_001382972.1; NP_001382973.1
- Function
- Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
MHC Tetramer Products for Cancer
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0930MZ | Recombinant Mouse Anti-Human MTAP Antibody | ICC/IF | Mouse antibody |
| VS3-FY966 | Recombinant Rabbit Anti-MTAP Antibody (VS3-FY966) | IA | Rabbit IgG |
| VS3-FY2810 | Rabbit Anti-MTAP Recombinant Antibody (VS3-FY2810) | WB, IP | Rabbit IgG |
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