Rabbit Anti-MTAP Recombinant Antibody (clone R07-5A0)
CAT#: VS3-FY2040
The R07-5A0 recombinant monoclonal antibody is capable of recognizing human MTAP. It cross-reacts with mouse. The antibody has the positive detection of MTAP protein by western blot analysis of MTAP in 3T3 lysates and western blot analysis of MTAP in A549, MCF-7, U251, HL-60 lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46915/1916_C10_25_cr5c99f3ba8408a_selected.jpg
Normal Tissue
Figure 2 IHC staining of human endometrium
Immunohistochemical staining of human endometrium shows moderate nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/80036/170588_B_5_3_selected.jpg
Normal Tissue
Figure 3 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong nuclear and cytoplasmic positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/80037/170596_B_6_1_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/80037/170596_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/80037/170596_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/80037/170596_A_9_5.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/80037/170596_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000099810-MTAP
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WB
Figure 1 Anti-MTAP Antibody (VS3-FY2040) in WB.
The MTAP expression in 3T3 lysates were detected using the anti-MTAP by western blot assay.
WB
Figure 2 Anti-MTAP Antibody (VS3-FY2040) in WB.
The MTAP expression in A549, MCF-7, U251, HL-60 lysates were detected using the anti-MTAP by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human MTAP
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse MTAP
- Species Reactivity
- Human, Mouse
- Clone
- R07-5A0
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 31 kDa, while the observed molecular weight stands at 31 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Methylthioadenosine Phosphorylase; S-Methyl-5-Thioadenosine Phosphorylase; 5-Methylthioadenosine Phosphorylase; MTA Phosphorylase; MTAPase; MSAP; Epididymis Luminal Protein 249; MeSAdo Phosphorylase
- Gene ID
- 4507
- UniProt ID
- Q13126
- Sequence Similarities
- Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys40 and Lys71 (NX_Q13126 [NX_Q13126-1])
Modification sites at PhosphoSitePlus (Q13126)
Glycosylation from GlyGen (Q13126) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001382969.1; NP_001382970.1; NP_001382971.1; NP_001382972.1; NP_001382973.1
- Function
- Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MTAP"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0930MZ | Recombinant Mouse Anti-Human MTAP Antibody | ICC, IF | Mouse antibody |
| VS3-FY966 | Recombinant Rabbit Anti-MTAP Antibody (VS3-FY966) | IA | Rabbit IgG |
| VS3-FY2810 | Rabbit Anti-MTAP Recombinant Antibody (VS3-FY2810) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1583 | PE-B*41:04/Human MTAP (HEEAVSVDRVL) MHC Tetramer | FCM | |
| MHC-LC1584 | APC-B*41:04/Human MTAP (HEEAVSVDRVL) MHC Tetramer | FCM | |
| MHC-LC1585 | BV421-B*41:04/Human MTAP (HEEAVSVDRVL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2314 | Hi-Affi™ Recombinant Rabbit Anti-MTAP Monoclonal Antibody (DS2314AB) | WB, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4557 | Anti-MTAP Immunohistochemistry Kit | IHC |
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