Rabbit Anti-MTAP Recombinant Antibody (clone R07-5A0) (CAT#: VS3-FY2040)
The R07-5A0 recombinant monoclonal antibody is capable of recognizing human MTAP. It cross-reacts with mouse. The antibody has the positive detection of MTAP protein by western blot analysis of MTAP in 3T3 lysates and western blot analysis of MTAP in A549, MCF-7, U251, HL-60 lysates.
Specific Inquiry
WB
Figure 1 Anti-MTAP Antibody (VS3-FY2040) in WB.
The MTAP expression in 3T3 lysates were detected using the anti-MTAP by western blot assay.
WB
Figure 2 Anti-MTAP Antibody (VS3-FY2040) in WB.
The MTAP expression in A549, MCF-7, U251, HL-60 lysates were detected using the anti-MTAP by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human MTAP
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse MTAP
- Species Reactivity
- Human, Mouse
- Clone
- R07-5A0
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 31 kDa, while the observed molecular weight stands at 31 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Methylthioadenosine Phosphorylase; S-Methyl-5-Thioadenosine Phosphorylase; 5-Methylthioadenosine Phosphorylase; MTA Phosphorylase; MTAPase; MSAP; Epididymis Luminal Protein 249; MeSAdo Phosphorylase
- Gene ID
- 4507
- UniProt ID
- Q13126
- Sequence Similarities
- Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys40 and Lys71 (NX_Q13126 [NX_Q13126-1])
Modification sites at PhosphoSitePlus (Q13126)
Glycosylation from GlyGen (Q13126) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001382969.1; NP_001382970.1; NP_001382971.1; NP_001382972.1; NP_001382973.1
- Function
- Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
MHC Tetramer Products for Cancer
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0930MZ | Recombinant Mouse Anti-Human MTAP Antibody | ICC/IF | Mouse antibody |
| VS3-FY966 | Recombinant Rabbit Anti-MTAP Antibody (VS3-FY966) | IA | Rabbit IgG |
| VS3-FY2810 | Rabbit Anti-MTAP Recombinant Antibody (VS3-FY2810) | WB, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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