Human Anti-MVP Recombinant Antibody (clone MVP-φ4) (CAT#: FAMAB-0097JF)
Recombinant human monoclonal antibody specially binds to MVP. This anti-MVP antibody MVP-φ4 can be a valuable tool in elucidating the contribution(s) of major vault protein/vaults to normal physiology and cellular drug resistance mechanisms.
Specific Inquiry
WB
Figure 1 Cell lysates were prepared for western blotting with an antibody against B7-H3, MVP, total MEK, MEK-p, and β-actin(or tubulin) was used as a loading control.
The number of positive-stained cells was calculated.
Liu, Z., Zhang, W., Phillips, J. B., Arora, R., McClellan, S., Li, J., ... & Tan, M. (2019). Immunoregulatory protein B7-H3 regulates cancer stem cell enrichment and drug resistance through MVP-mediated MEK activation. Oncogene, 38(1), 88-102.
IHC
Figure 2 Tissue microarrays were stained with MVP antibodies.
The intensity of the positive staining was scored.
Liu, Z., Zhang, W., Phillips, J. B., Arora, R., McClellan, S., Li, J., ... & Tan, M. (2019). Immunoregulatory protein B7-H3 regulates cancer stem cell enrichment and drug resistance through MVP-mediated MEK activation. Oncogene, 38(1), 88-102.
IF
Figure 3 B7-H3 overexpression disrupts polarity and acinar structure with increased invasion potential.
Cells were seeded in 3D Matrigel culture and grown for 8 days.
Liu, Z., Zhang, W., Phillips, J. B., Arora, R., McClellan, S., Li, J., ... & Tan, M. (2019). Immunoregulatory protein B7-H3 regulates cancer stem cell enrichment and drug resistance through MVP-mediated MEK activation. Oncogene, 38(1), 88-102.
FC
Figure 4 MEK activation is required for B7-H3 induced breast stem cells and acinar disruption.
MCF-10A-B7H3 cells were transfected with scramble and MEK1/2 siRNA.
Liu, Z., Zhang, W., Phillips, J. B., Arora, R., McClellan, S., Li, J., ... & Tan, M. (2019). Immunoregulatory protein B7-H3 regulates cancer stem cell enrichment and drug resistance through MVP-mediated MEK activation. Oncogene, 38(1), 88-102.
Specifications
- Host Species
- Human
- Derivation
- Human phage display Fab library
- Type
- Human IgG
- Specificity
- Human MVP
- Species Reactivity
- Human
- Clone
- MVP-φ4
- Applications
- WB, FC, IHC, IF
- Related Disease
- Tumors
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The MVP antibody has been reported in applications of Western Blot, Flow Cytometry, Immunohistochemistry, Immunofluorescence.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone MVP-φ4"
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3113z | Mouse Anti-MVP Recombinant Antibody (clone 10A5) | WB, FC, ICC, IF, ICC, IF | Mouse IgG1, κ |
| MOB-0078CT | Recombinant Mouse anti-Human MVP Monoclonal antibody (MSQ-67) | FC, ICC, IHC-Fr, IHC-P | |
| MRO-1046-CN | Recombinant Rabbit Anti-MVP Monoclonal Antibody (JM74-73) | WB, IHC | Rabbit IgG |
| VS3-WK612 | Rabbit Anti-MVP Recombinant Antibody (clone JM74-73) | WB, ELISA | Rabbit IgG |
| VS3-FY991 | Recombinant Rabbit Anti-MVP Antibody (clone R09-3D5) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2330 | Hi-Affi™ Recombinant Rabbit Anti-MVP Monoclonal Antibody (DS2330AB) | WB, ICC/IF, FC, IHC-P | IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0097JF-F(E) | Human Anti-MVP Recombinant Antibody (clone MVP-φ4); Fab Fragment | IP, IHC, WB, ELISA, FuncS | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0097JF-S(P) | Human Anti-MVP Recombinant Antibody (clone MVP-φ4); scFv Fragment | IP, IHC, WB, ELISA, FuncS | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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