Rabbit Anti-MYL2 Recombinant Antibody (clone R08-2D2) (CAT#: VS3-XY3441)
This is a recombinant rabbit antibody targeting the MYL2 (Myosin light chain 2), which plays a role in heart development and function. It recognizes and binds to human MYL2. It also has cross-reactivity against mouse and rat MYL2. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin and Immunoprecipitation. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin and Immunoprecipitation is 1/500-1/1000, 1/50-1/100 and 1/20, respectively.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19763/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19763/if_selected.jpg
Figure 2 IHC staining of human skeletal muscle
(Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19763/144110_B_3_6_selected.jpg
(Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19763/144110_B_3_6_selected.jpg
Figure 3 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_A_8_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_A_8_3.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_A_7_5.jpg
(Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_A_7_5.jpg
Figure 5 Heart muscle
(Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_B_4_6.jpg
(Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_B_4_6.jpg
Figure 6 Cerebral cortex
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_B_7_5.jpg
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_B_7_5.jpg
Figure 7 RNA cell line category: Cell line enhanced (EFO-21, HSkMC, U-2 OS)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111245-MYL2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111245-MYL2
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MYL2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-2D2
- Applications
- WB, IHC-P, IP
- Related Disease
- Cardiomyopathy, Familial Hypertrophic, 10; Myopathy, Myofibrillar, 12, Infantile-Onset, With Cardiomyopathy
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IP: 1/20
Target
- Alternative Names
- MLC2; CMH10; MFM12; MLC-2; MLC-2v; MLC-2s/v
- Gene ID
- 4633
- UniProt ID
- P10916
- Long Name
- Myosin light chain 2
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- N-terminus is methylated by METTL11A/NTM1.
Phosphorylated by MYLK3 and MYLK2; promotes cardiac muscle contraction and function. Dephosphorylated by PPP1CB complexed to PPP1R12B (By similarity).
The phosphorylated form in adult is expressed as gradients across the heart from endocardium (low phosphorylation) to epicardium (high phosphorylation); regulates cardiac torsion and workload distribution (PubMed:22426213).
- Protein Refseq
- NP_034991.3
- Function
- Contractile protein that plays a role in heart development and function (PubMed:10409661).
Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity) (PubMed:22426213, PubMed:16908724, PubMed:10409661).
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2241MZ | Recombinant Mouse Anti-Human MYL2 Antibody | WB | Mouse antibody |
| ZG-0297C | Mouse Anti-MYL2 Recombinant Antibody (ZG-0297C) | WB, ELISA | Mouse IgG |
| VS3-CJ1021 | Rabbit Anti-MYL2 Recombinant Antibody (VS3-CJ1021) | WB, IHC, IP | Rabbit IgG |
| VS3-XY1129 | Mouse Anti-MYL2 Recombinant Antibody (clone 7C9) | ELISA, WB | Mouse IgG1 |
| VS-0223-WK47 | Rabbit Anti-MYL2 Recombinant Antibody (VS-0223-WK47) | WB, ICC, IHC-P | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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