Rabbit Anti-MYL2 Recombinant Antibody (VS3-CJ1021)
CAT#: VS3-CJ1021
This product is a rabbit antibody that recognizes mouse, rat, and zebrafish MYL2.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19763/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human skeletal muscle
Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19763/144110_B_3_6_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_A_8_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_A_7_5.jpg
Normal Tissue
Figure 5 Heart muscle
Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_B_4_6.jpg
Normal Tissue
Figure 6 Cerebral cortex
Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39262/109225_B_7_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (EFO-21, HSkMC, U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111245-MYL2
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse, Rat, Zebrafish MYL2
- Species Reactivity
- Mouse, Rat, Zebrafish
- Applications
- WB, IHC, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunohistochemistry, Immunoprecipitation.
Target
- Alternative Names
- MLC-2; Mlc2v; Mylpc; MLC-2v; Gm32672; MLC-2s/v
- Gene ID
- 17906
- UniProt ID
- P51667
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- N-terminus is methylated by METTL11A/NTM1.
Phosphorylated by MYLK3 and MYLK2; promotes cardiac muscle contraction and function. Dephosphorylated by PPP1CB complexed to PPP1R12B (By similarity).
The phosphorylated form in adult is expressed as gradients across the heart from endocardium (low phosphorylation) to epicardium (high phosphorylation); regulates cardiac torsion and workload distribution (PubMed:22426213).
- Protein Refseq
- NP_034991.3
- Function
- Contractile protein that plays a role in heart development and function (PubMed:10409661).
Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity) (PubMed:22426213, PubMed:16908724, PubMed:10409661).
During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (PubMed:9422794).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MYL2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2345 | Hi-Affi™ Recombinant Rabbit Anti-MYL2 Monoclonal Antibody (DS2345AB) | WB, IHC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2053-CN | Rabbit Anti-MYL2 Polyclonal Antibody (MRO-2053-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS-0223-WK47 | Rabbit Anti-MYL2 Recombinant Antibody (VS-0223-WK47) | WB, ICC, IHC-P | Rabbit IgG |
| VS3-FY994 | Recombinant Rabbit Anti-MYL2 Antibody (clone R07-7C5) | WB, IHC-P, IP | Rabbit IgG |
| VS3-XY3441 | Rabbit Anti-MYL2 Recombinant Antibody (clone R08-2D2) | WB, IHC-P, IP | Rabbit IgG |
| VS13-YC760 | Rabbit Anti-MYL2 Recombinant Antibody (VS13-YC760) | WB, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1420 | Anti-MYL2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4641 | Anti-Mouse MYL2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4640 | Anti-Human MYL2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4642 | Anti-Rat MYL2 Immunohistochemistry Kit | IHC |
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